电磁场扰动下的细胞系统鲁棒性  

Robustness of Cell System Under Electromagnetic Field Disturbance

作　　者：郑秀秀[1] 包家立[1] 朱朝阳[1] 

机构地区：[1]浙江大学医学院浙江省生物电磁学重点实验室生物物理与医学工程研究组,杭州310058

出　　处：《高电压技术》2014年第12期3837-3845,共9页High Voltage Engineering

基　　金：国家自然科学基金(61071060)~~

摘　　要：生物鲁棒性是生物系统抵抗外界扰动或内部参数摄动引起系统行为变化的一种能力。电磁场是一种外界物理因素,可以对生物体产生影响。为了建立一种电磁场扰动下生物系统稳定鲁棒性的研究方法,以Lyapunov理论为基础,用电磁场细胞暴露系统实时记录了在磁感应强度B为0、0.09、0.38、0.76、7.33、14.78 m T的电磁场暴露下细胞内活性氧自由基（ROS）和钙离子（Ca2＋）的含量,分析了电磁场扰动前的细胞状态稳态点和稳定域,以及电磁场扰动后的状态转移和稳定鲁棒域。结果表明：（1）细胞无扰时ROS的稳态点是46.157~120.913,Ca2＋的稳态点是25.430~55.686,ROS的稳定域半径是1.688~10.278,Ca2＋的稳定域半径是2.782~13.345;（2）B〈7.33 m T的电磁场扰动没有提高胞内ROS含量,B为14.78 m T的电磁场扰动可阶跃性提高胞内ROS含量,B为0和0.09 m T的电磁场扰动没有提高胞内Ca2＋含量,B为0.38 m T的电磁场扰动可持续性提高胞内Ca2＋含量。结论是：（1）无电磁场扰动时,细胞内ROS和Ca2＋状态可以保持稳定;（2）电磁场扰动在较高水平（如B为14.78 m T）时,才能改变细胞内ROS和Ca2＋的状态;（3）细胞的鲁棒域与电磁场的磁感应强度无关。Biological robustness describes an ability of a life system to resist behavior change caused by external disturbances or internal parameter perturbation. To study the steady-state biological robustness of a life system being exposed to electromagnetic fields（EMFs）, we measured the concentrations of reactive oxygen species（ROS） and intracellular calcium（Ca2＋） in hippocampal neurons exposed to EMFs of various intensity, namely 0, 0.09, 0.38, 0.76, 7.33, 14.78 m T, in an EMF exposure system in real-time, and analyzed the two parameters＇ steady points of states and steady domains before EMF disturbances, as well as their state transitions and robustness domains after the EMF exposure. Experimental results show that：（1） in the absence of EMF disturbance, the steady points are between 46.157 and 120.913 for ROS, and between 25.430 and 55.686 for Ca2＋, while the radius of stable domain is between 1.688 and 10.278 for ROS, and between 2.782 and 13.345 for Ca2＋.（2） The ROS level is not affected by EMFs with intensity below 7.33 m T, but increased with step response when the EMF＇s intensity is 14.78 m T; the Ca2＋ level is not affected by EMFs with intensity of 0 and 0.09 m T, but increased continuously with EMF disturbance of 0.38 m T. Some conclusions are drawn：（1） The states of intracellular Ca2＋ and ROS can keep stable in the absence of EMF disturbance;（2） the states can be changed by disturbances of stronger EMF, such as 14.78 m T;（3） the robust domain of hippocampal neurons is not related to the intensity of EMF disturbances.

关 键 词：极低频电磁场 ELF-EMFs 生物鲁棒性 细胞 LYAPUNOV稳定性 钙离子 活性氧自由基 

分 类 号：R594.8[医药卫生—内科学]