一株异养硝化细菌的分离鉴定及其脱氮效果  

Isolation Identification of a Heterotrophic Nitrifier and Its Efficiency of Nitrogen Removal

作　　者：尹丽[1] 肖小雨[1] 梁亮亮[1] 贺根和[1] 

机构地区：[1]井冈山大学生命科学学院,江西吉安343009

出　　处：《环境科学与技术》2014年第12期25-30,共6页Environmental Science & Technology

基　　金：国家科技支撑计划项目(2012BAC11B04-03);井冈山大学博士启动基金项目资助(JZB1307)

摘　　要：该研究从富营养化景观水体中分离出40株细菌,利用PCR-RFLP(限制性片段长度多态性)技术确定了11种不同的基因型,对11种不同基因型菌株氨氮(NH4+-N)和总氮(TN)的去除效果进行研究,发现其中1株菌在24 h内NH4+-N的去除率达87.7%,TN去除率达81.7%,而且该菌从培养体系中完全去除的氮明显多于同化到细胞内的氮。进一步对该菌进行分子鉴定,确定了其种属特征,并命名为Klebsiella sp.HLNR02。利用菌株HLNR02处理模拟富营养化废水,结果发现其在48 h内对模拟废水中的NH4+-N和TN去除率达85.3%和77.3%,C/N比会影响菌株的解氮效果。This paper reported on a laboratory study,in which 40 strains of bacteria were isolated from a scenic water body that had been eutrophicated,and 11 different genotypes were identified using PCR-RFLP（Restriction Fragment Length Polymorphism）,whose effects of removing ammonia nitrogen（NH4＋-N） and TN were studied. Findings of the study suggested that one strain,among others,could remove 87.7% of NH4＋-N and 81.7% of TN within 24 h respectively;moreover,the nitrogen which was removed from the inoculation system was higher than the assimilated into the cell. This strain was then identified as Klebsiella sp. and designated as HLNR02. When HLNR02 was used to remove nitrogen from the simulated eutrophicated water sample,removals of NH4＋-N up to 85.3% and TN 77.3% were obtained within 48 h. In addition,it was found the ratio C/N would affect nitrogen removing rate.

关 键 词：异养硝化 PCR-RFLP 模拟富营养化废水 氨氮 总氮 

分 类 号：X172[环境科学与工程—环境科学] Q939.9[生物学—微生物学]