角化棘皮瘤与高分化鳞状细胞癌细胞周期和细胞凋亡相关蛋白表达的研究  

The research on expression of cell cycle and apoptosis related protein in keratoacanthoma and well - dif- ferentiated squamous cell carcinoma

作　　者：刘冰梅[1] 杨晶[1] 邢荣贵[1] 李丽丽[2] 赵丽萍[1] 乔适[1] 尤海燕[1] 

机构地区：[1]黑龙江省医院皮肤科南岗院区哈尔滨150001 [2]齐齐哈尔市第一医院皮肤科

出　　处：《实用肿瘤学杂志》2014年第6期485-491,共7页Practical Oncology Journal

基　　金：黑龙江省自然科学基金项目（D201278）;哈尔滨市科技创新人才（2012RFXYS047）

摘　　要：目的：探讨细胞周期与细胞凋亡相关蛋白p53、bcl－2、Ki－67和细胞凋亡在角化棘皮瘤（Keratoacanthoma，KA）和高分化鳞状细胞癌（Well－differentiated squamous cell carcinoma ，WDSCC）中的表达及其意义。方法应用免疫组化技术和末端特异性DNA标记技术检测44例角化棘皮瘤、20例高分化鳞状细胞癌标本中p53、bcl－2、Ki－67和细胞凋亡的表达情况。结果 KA增生期、成熟期和消退期p53阳性表达率分别为：22．23％、26．18％和6．52％，低于WDSCC 41．82％。 KA各期分别与WDSCC阳性率比较，差异有统计学意义；Ki－67的表达强度和模式同p53相似，p53表达率与Ki－67表达率呈正相关（ r＝0．986，P＜0．001）；KA中仅1例基底层见bcl－2弱阳性表达，WDSCC中2例表达呈弱阳性；KA的平均凋亡率（21．72％）明显高于WDSCC（9．93％），两者比较差异有统计学意义，细胞凋亡率与增殖率（Ki－67）的表达呈负相关（r＝－0．824，P＜0．001）。结论增生和凋亡同时存在于KA中，早期增生占优势，而消退期凋亡占优势，最终导致KA的自然消退。 p53、Ki－67以及细胞凋亡的表达对临床鉴别KA与WDSCC有一定参考价值。Objective To explore the expressions and significances of p 53、bcl-2、Ki-67 and apopto-sis in keratoacanthoma（KA）and well differentiated squamous cell carcinoma （WDSCC）.Methods The expres-sions of p53、bcl-2、Ki-67 were detected by immunohistochemical technique in 44 cases of KA and 20 cases of WDSCC.Apoptosis was identified by using terminal deoxynucleotidyl transfers （ TdT） -mediated dUTP -biotin nick and labelling（TUNEL）method.Results The positive expressive rates of p53 in proliferative、mature and re-gressive of KA were 22.23%、26.18% and 6.52%, which was lower than the positive rate of WDSCC （41.82%）.Significant differences were found between every period of KA with WDSCC in p 53 expression;The intensity of expression and pattern were similar in KA of Ki -67 and p53.There were a positive correlation be-tween p53 and Ki-67 expression rate（r=0.986,P〈0.001）.The bcl-2 staining showed weak expression in 1 case in WDSCC and 2 cases in KA ,but only a few positive tumor cells was limited to basal cell layer in KA .The average apoptosis rate in KA was 21.72%,which was apparently higher than in WDSCC （9.925%）.There were a negative correlation between the apoptosis rate and proliferation rate of Ki -67（r=-0.824,P〈0.001）.Con-clusion The proliferation and apoptosis in KA can coexist .However ,the apoptosis will occupy a dominance posi-tion in regressive phase ,which results in the regression of tumor .The expressions of p53、Ki-67 and apoptosis would be a certain significance in differentiated KA and WDSCC .

关 键 词：角化棘皮瘤 鳞状细胞癌 p53 bcl-2 KI-67 

分 类 号：R739.5[医药卫生—肿瘤]