C2-神经酰胺对人间皮瘤细胞凋亡的诱导作用  

C2 - ceramide induced apoptosis of malignant mesothelioma cells in vitro

作　　者：张天华[1] 李永春[2] 李宪伟[1] 迟德财[1] 

机构地区：[1]哈尔滨医科大学附属第二医院普外五科,哈尔滨150086 [2]牡丹江医学院红旗医院普外科

出　　处：《实用肿瘤学杂志》2014年第6期492-497,共6页Practical Oncology Journal

基　　金：黑龙江省留学归国科学基金项目（LC2009C17）

摘　　要：目的研究c2-神经酰胺对体外培养的人间皮瘤细胞的凋亡及其相关分子的影响。方法采用CCK-8法检测不同剂量的c2-神经酰胺作用于间皮瘤后不同时间的细胞增殖情况；采用DiffQuik染色观察细胞形态学上的凋亡改变。用FluorescentAssay法检测间皮瘤细胞的Caspase-3活性；通过Westernblot检测细胞凋亡发生过程中的关键蛋白的表达。结果c2-神经酰胺对间皮瘤细胞的抑制呈现剂量依从性和时间依从性。DiffQuik染色观察到凋亡小体。C2-神经酰胺能增加间皮瘤细胞Caspase-3的活性。C2-神经酰胺可使间皮瘤细胞Bax蛋白、磷酸化JNK蛋白增加、磷酸化ERKI／2蛋白下降。结论c2-神经酰胺可在体外诱导人间皮瘤细胞凋亡，并对其凋亡通路中的关键分子有影响，发挥了一定的抗肿瘤作用。Objective In this research,the effects of exogenous C2 - ceramide on the induction of apop- tosis of mesothelioma cells in vitro and related important proteins are investigated. Methods Mesothelioma cells were treated with various doses of C2 - ceramide for different duration. Cell viability were analyzed by cell count- ing kit -8 assay. Morphological changes of apoptosis of mesothelioma cells were observed by Diff Quik staining. Apoptosis of mesothelioma cells was also detected by Caspase - 3 assay and lactate dehydrogenase （LDH） assay. Related important proteins involved in the signal transduction of apoptosis were detected by western blot. Results In vitro, C2 - ceramide demonstrated a dose - and time - dependent inhibition of cell proliferation. Apoptotic bodies were observed by Diff Quik staining. The antiproliferative effect of 80 μm C2 - ceramide was paralleled with an increase in Caspase -3 activity. LDH assay showed that C2 - ceramide at a concentration of 80μm signifi- candy promoted cells death. After treated by C2 - ceramide, the expressions of Bax and the phosphorylated JNK in mesothelioma cells were increased, however, the expression of phosphorylated ERK1/2 kinase was decreased. Conclusion Our results indicate that C2 - ceramide induced apoptosis of malignant mesothelioma cells in vitro. This anti - tumor affect is achieved by adjusting related important proteins.

关 键 词：神经酰胺 间皮瘤 细胞凋亡 

分 类 号：R73[医药卫生—肿瘤]