天然抗氧化剂α-硫辛酸对血管性痴呆大鼠线粒体的保护作用  

Protective effects of natural antioxidant alpha-lipoic acid on mitochondria in a rat model of vascular dementia

作　　者：赵冉冉[1,2] 徐晓臣[3] 徐飞[4] 刘存祥 檀国军[1] 李彦改[3] 陈朝旺[2] 

机构地区：[1]河北医科大学第二医院神经内科,石家庄050071 [2]邯郸市第一医院急诊科 [3]邯郸市第一医院神经内科 [4]邯郸市第一医院泌尿科 [5]邯郸市魏县中医院神经外科

出　　处：《山西医科大学学报》2014年第12期1136-1139,共4页Journal of Shanxi Medical University

基　　金：河北省科技厅科研基金资助项目(11276425)

摘　　要：目的探讨α-硫辛酸(ALA)对血管性痴呆(VD)大鼠的神经保护作用及机制。方法 40只健康成年雄性Wistar大鼠行双侧颈总动脉结扎以制备血管性痴呆大鼠模型。动物随机分为假手术组、血管性痴呆组、α-硫辛酸50 mg/(kg·d)干预血管性痴呆组(VD+ALA)和α-硫辛酸50 mg/(kg·d)干预假手术组(sham+ALA)。利用Morris水迷宫观测各组动物学习记忆能力,流式细胞仪测定各组动物脑组织线粒体膜电位(mitochondrial membrane potential,MMP)、活性氧自由基(reactive oxygen species,ROS)的水平,脑组织超氧化物歧化酶(superoxide dismutase,SOD)含量。结果与假手术组相比,血管性痴呆大鼠脑组织中MMP下降,ROS含量升高,SOD含量下降;α-硫辛酸干预血管性痴呆组与血管性痴呆组相比大鼠学习记忆能力改善,脑组织MMP明显升高,ROS明显下降,SOD含量增加。结论线粒体功能障碍和氧化应激损伤在VD发病中有非常重要的作用,ALA能够保护脑线粒体功能,减轻氧化应激损伤,改善认知功能。Objective To explore the neuroprotective effects and mechanisms of alpha-lipoic acid （ALA） in a rat model of vascular dementia（VD）.Methods Bilateral common carotid arteries occlusion（BCCAO） was applied in adult male Wistar rats to establish VD model.Animals were randomly assigned into four groups：sham （sham-operated） group,VD group,VD ＋ ALA group,sham ＋ ALA group.The model rats were intraperitoneally injected with normal saline in VD group,and 50 mg/kg alpha-lipoic acid in VD ＋ ALA group.The sham-operated rats were intraperitoneally injected with 50 mg/kg alpha-lipoic acid in sham ＋ ALA group.Learning and memory deficits were tested by Morris water maze.Flow cytometry was applied to detect mitochondrial membrane potential and the level of reactive oxygen species（ROS） in brain tissues.Spectrophotometry was used to determine the concentrations of superoxide dismutase（SOD） in brain tissues.Results In brains of the VD rats,mitochondrial membrane potential significantly decreased,ROS significantly increased,and SOD significantly decreased.However,ALA significantly improved cognitive deficits,protected mitochondrial membrane potential,significantly decreased ROS and increased superoxide dismutase.Conclusion Mitochondrial damage and oxidative stress play important roles in the pathogenesis of vascular dementia.ALA could protect the mitochondrial function,ameliorate the oxidative damage and improve the cognitive function.

关 键 词：血管性痴呆 氧化应激 线粒体 大鼠 

分 类 号：R742[医药卫生—神经病学与精神病学]