肝癌细胞p53、p16及细胞周期蛋白D1表达的时间生物学对比  

Chronobiologic contrast of expression of p53, p16, and cyclin Dl in hepatocarcinoma cells

作　　者：余万霰[1] 张永虹[1] 蔡勇[2] 周晓军[3] 

机构地区：[1]江西医学院附属第二医院,南昌330006 [2]江西省肿瘤医院病理科 [3]江西医学院预防医学系卫生统计教研室

出　　处：《中华肝脏病杂志》2002年第4期253-255,共3页Chinese Journal of Hepatology

基　　金：国家自然科学基金(3986003)

摘　　要：目的 对比肝癌细胞中p53、p16和细胞周期蛋白D1（Cyclin　D1）表达强度的时段特征。方法 按年周期3个时段采集肝癌组织标本,每1个时段20例,均为肝细胞型肝癌及Edmondson-Steinet分级控制在Ⅱ～Ⅲ级内,用免疫组织化学S-P法进行p53、p16和Cyclin D1蛋白表达实验,表达强度按等级数据用秩和检验方祛进行时段对比。结果 p16表达出现时段差异有显著性（H=10.34,P<0.05）,即4～7月份为表达高峰期。结论 肝细胞癌变及其生长的基因调控的时间生物学机制,p16可能起到重要作用。Objective To contrast the feature of different periods of p53, p16, and cyclin D1 expression in cells of hepatocarcinoma. Methods Sixty hepatocarcinoma specimens were collected and divided into three periods in one yearly cycle with each period of 20 cases. Hepatocellular carcinoma was documented in all cases by pathomorphological classification. Edmondson-Steinet grading belonged to Ⅱ -Ⅲ stages. The expression of gene proteins of p53, p16, and cyclin Dl was determined by immunohistochemical assay (S-P method). The expressive intensity was analysed by the rank test. Results p16 expression showed significant difference of periods (H=10.334, P<0. 05). Arpil-July was the highest expressive period. Conclusions p16 may take an important role in the chronobiologic mechanism of gene control of hepatocyte canceration and hepatocarcinoma cell growth.

关 键 词：肝细胞癌 P53基因 P16基因 时间生物学 细胞周期蛋白D1 免疫组织化学S-P法 

分 类 号：R737.5[医药卫生—肿瘤]