改良掺食法建立苯巴比妥成瘾大鼠模型  

Establishement of phenobabital addiction rat model by modified drug admixed food method

作　　者：韩彬[1] 王国俊[1] 傅萍[2] 叶云[1] 

机构地区：[1]泸州医学院附属医院药剂科,四川泸州646000 [2]四川省食品药品检验所,成都611731

出　　处：《重庆医学》2014年第36期4924-4927,共4页Chongqing medicine

基　　金：四川省中药管理局基金资助(12086)

摘　　要：目的研究用掺食法建立苯巴比妥成瘾大鼠模型的方法。方法选用大鼠为实验动物,采用掺食法造模,苯巴比妥起始剂量75mg/kg,逐日递增剂量,经45d,模型分别采用文献报道的催促戒断法及自然戒断法加以检验,判断造模是否成功。结果掺食法催促戒断组的惊厥率明显高于对照组(P<0.01);掺食法自然戒断组的戒断症状评分明显高于对照组(P<0.05);两个戒断组的体质量减轻程度均大于对照组(P<0.05);造模组脑切片显示明显的细胞凋亡改变。结论掺食法建立苯巴比妥成瘾大鼠模型简单可行。Objective To establish the phenobarbital addiction rat model by the modified drug‐admixed‐food（DAF） method . Methods Rats were selected as the experimental animal .The modified DAF method was adopted to construct the model .The initial dose of phenobarbital 75 mg/kg was given mixed and the dase was gradually increased day by day ,which continued for 45 d .The es‐tablished model was verified by the precipitated withdrawal method and the natural withdrawal method for judging whether the model construction succeeding .Results The convulsion rate in the DAF precipitated withdrawal group was higher than that in the control group（P〈 0 .01） ;the withdrawal symptoms scores in the DAF natural withdrawal group were higher than those in the con‐trol group（P〈 0 .05） ;the relieving degree of body mass in the two withdrawal groups was greater than that in the control group （P〈 0 .05） .The brain section in the mode group revealed obvious cellular apoptotic change .Conclusion DAF is simple and feasible for establishing the phenobarbital addiction rat model .

关 键 词：掺食法 苯巴比妥成瘾 大鼠模型 

分 类 号：R-332[医药卫生] R749.61