胃癌组织高迁移率族蛋白B1的表达及与临床病理特征的关系  

Expression of HMGB1 and the relationship with clinicopathological characteristics of gastric carcinoma

作　　者：姜飚[1] 靳猛[1] 张伟[1] 李强[2] 

机构地区：[1]济宁医学院附属医院,山东济宁272029 [2]天津医科大学附属肿瘤医院,天津300070

出　　处：《济宁医学院学报》2014年第6期407-410,共4页Journal of Jining Medical University

摘　　要：目的检测高迁移率族蛋白B1(HMGB1)在胃癌组织的表达,分析其与胃癌临床病理特征的关系。方法 112例胃癌及癌旁组织标本应用免疫组织化学法、反转录PCR、Western-blot检测HMGB1的表达、定量,应用统计学方法分析其与胃癌临床病理特征关系。结果免疫组织化学染色显示:HMGB1阳性颗粒主要位于细胞核;反转录PCR、Western-blot显示:胃癌组织中的表达水平明显高于癌旁组织和正常胃组织(P<0.01),HMGB1过度表达与胃癌的分化程度、组织学的类型及有无淋巴结转移关系密切。结论 HMGB1在胃癌组织中高表达与胃癌的临床病理特征密切相关,推测其过表达可能与胃癌的侵袭、转移有关。Objective To investigate the significance of high mobility group protein B1 （HMGB1） in gastric carcinoma （GC） and its relationship with clinicopathological characteristics .Methods 112 cases of GC specimens and tumor‐adjacent tissues were collected .The expression of HMGB1 was tested with immunohistochemistry ,re‐verse transcription‐polymerase chain reaction （RT‐PCR ） and Western‐blot .Clinicopathological characteristics of GC patients were also analysed .The relationship between HMGB1 and the pathological characteristics of GC was analysed statistically .Results Immunohistochemistry data demonstrated that HMGB1 protein was predominantly localized in the nuclei of GC .Results of RT‐PCR and Western‐blot demonstrated that the expression of relative HMGB1 mRNA and protein in the tissue of GC were higher than that in tissue adjacent to carcinoma and normal gastric tissue （ P〈0 .01 ） .Overexpression of HMGB1 was significantly correlated with histological type ,differentiation and lymph node metastasis of tumor .Conclusion Overexpression of HMGB1 was related to some clinicopatho‐logical characteristics of GC ,and HMGB1 may promote invasion and metastasis of GC .

关 键 词：HMGB1 胃癌 临床病理 

分 类 号：R735.2[医药卫生—肿瘤]