人工培养长座线虫草子实体及其菌丝体的挥发性成分分析  

Volatile components in mycelia and fruiting bodies of the artificial culture of Ophiocordyceps longissima

作　　者：张颖裕 刘洋[1] 李扬眉[1] 张龙娃[1] 李春如[1] 

机构地区：[1]安徽农业大学微生物防治省重点实验室,合肥230036

出　　处：《安徽农业大学学报》2014年第6期994-998,共5页Journal of Anhui Agricultural University

基　　金：国家自然科学基金(30570004);国家高技术研究发展计划项目(863计划)(2007AA021506)共同资助

摘　　要：首次采用同时蒸馏萃取法(SDE)提取了人工培育的长座线虫草子实体及摇瓶菌丝体的挥发性成分,并用气相色谱质谱联用仪(GC-MS)进行了分析。结果表明,不同培养方式对长座线虫草挥发性物质的数目和含量有较大影响,对挥发性成分的类型影响较大,液体摇瓶菌丝体挥发性物质种类主要有:酮类、醇类、烷烃类、烯烃类、醌类和醚类化合物;子实体中挥发性物质种类主要有:醇类、醛类和酚类。液体摇瓶菌丝体鉴定出20种挥发性物质,以1-辛烯-3-醇(37.70%)为主,人工培养子实体鉴定出6种挥发性物质,以1-辛烯-3-醇(51.93%)和2,6-二叔丁基对甲苯酚(38.37%)为主。2种人工培养物挥发性物质的共有成分为共有成分1-辛烯-3-醇、2,6-二叔丁基对甲苯酚在菌丝体中占51.66%,在子实体的挥发性成分中占90.30%。The volatile compounds were extracted from the mycelia and fruiting bodies of the artificial cul- ture of OphiocordycePs longissima using simultaneous distillation and extraction （SDE） method. Their volatile components were analyzed using gas chromatography-mass spectrometry （GC-MS）. The results showed that cul- ture conditions had great influence on the content and number of volatile eomponents; however, little impact on component classes was observed. The main volatile substances in mycelia were ketones, alcohols, alkenes, phe- nols and ethers, while alcohols, aldehydes, and phenols are main volatile compounds in fruiting bodies. Twenty compounds were detected in the mycelia liquid in which the content of 1-octene-3-alcohl （37.70%） was the high- est. Six compounds were identified from the artificial culture of the fruiting bodies with the content of 1-octene-3-alcohl （51.93%） and butylated hydroxytoluene （38.37%）being relatively high. The compounds of 1-octen-3-ol and butylated hydroxytoluene are two common constituents in both artificial cultures and their con- tents were accounted for 51.66% and 90.30% of the total volatile components in mycelia and fruiting body, re- spectively.

关 键 词：长座线虫草 菌丝体 子实体 挥发性成分 SDE GC-MS 

分 类 号：Q939.99[生物学—微生物学]