基于转录组序列的小麦ETS-SSR标记筛选与染色体定位  

Screening and Chromosomal Localization of EST-SSR Molecular Markers Based on Transcriptome Sequencing of Wheat

作　　者：杨会[1] 杨在君[1] 魏淑红[1] 廖明莉[1] 杨宇凤 王育伟[1] 王清海[1] 彭正松[1] 

机构地区：[1]西华师范大学西南野生动植物资源保护省部共建教育部重点实验室,四川南充637009

出　　处：《西华师范大学学报（自然科学版）》2014年第4期315-321,共7页Journal of China West Normal University(Natural Sciences)

基　　金：国家自然科学基金(31301319);四川省教育厅项目(12ZB325);四川省科技厅项目(2012HH0015)2)

摘　　要：通过小麦转录组序列分析,获得121 210个非重复序列(Unigenes),在10 672(8.8%)个Unigenes中搜索出1-6个重复基元的11 650条EST-SSR信息位点,筛选并设计出308条SSR引物,选取前30对引物进行合成.有效性扩增检测结果表明,20(66.7%)对引物有清晰条带.利用缺体-四体系共定位了14对引物,分别位于13条染色体的21个位点上.研究表明,利用小麦转录组中EST-SSR信息开发新的SSR标记是可行的,开发的新ESTSSR标记可有效用于小麦基因的定位和遗传多样性的分析等.121 210 unigenes were obtained by wheat transciptome sequencing, and 11 650 EST-SSR message items were screened from the 10 672 （8.8%） unigenes of wheat. 308 EST-SSR primers were designed,and the first 30 makers were selected for this study. Among them, 20 （66.7%）markers showed clear strip after effective amplifica- tion detection. Through a set of wheat nulli-tetrasomics, 14 EST-SSR markers were located on 21 loci of 13 chromo- somes. This study indicated that new SSR marker development using EST-SSR message items in wheat transciptome sequence is feasible. These new EST-SSR markers can be used in gene mapping and genetic diversity studies in wheat.

关 键 词：转录组测序 EST-SSR 小麦 

分 类 号：S512.1[农业科学—作物学]