乳酸作为EBPR进水碳源的可行性研究  

Feasibility study on enhanced biological phosphorus removal by using lactic acid as an influent carbon source

作　　者：龙建兵[1] 顾向阳[1] 

机构地区：[1]南京农业大学生命科学学院/农业部农业环境微生物工程重点开放实验室,江苏南京210095

出　　处：《南京农业大学学报》2015年第1期85-92,共8页Journal of Nanjing Agricultural University

基　　金：美国洛克菲勒基金项目(V200601);国家自然科学基金项目(50778089)

摘　　要：[目的]探索乳酸作为强化生物除磷(EBPR)进水碳源的可行性。[方法]首先采用厌氧-好氧型序批式生物反应器,以乳酸为唯一进水碳源驯化EBPR污泥(SBR-2),并与以乙酸为唯一进水碳源的EBPR污泥(SBR-1)对比;待SBR-2达到稳态运行后,在1个运行周期定期取样,测定混合液中化学需氧量(COD)、磷酸根、氨氮和硝氮等参数的周期变化,并与SBR-1比较;通过研究不同进水磷浓度对2个反应器除磷效率的影响,比较2种EBPR污泥的除磷能力。[结果]以乙酸为进水碳源的SBR-1和以乳酸为进水碳源的SBR-2分别经过8和31 d的驯化期实现了稳定的EBPR功能;与乙酸驯化的EBPR污泥类似,乳酸驯化所得的EBPR污泥也表现出典型聚磷菌(PAO)的代谢特征。以乳酸为碳源驯化而成的EBPR污泥其主要特征参数如有机酸吸收速率、磷释放速率和磷吸收速率均明显低于以乙酸为碳源驯化而成的EBPR污泥,但是由于其厌氧释放的磷浓度较低,结果仍可在相同的时间内完成磷的吸收。在进水磷酸根质量浓度小于等于12 mg·L-1时,以乳酸为进水碳源的反应器可以实现稳定高效的除磷效果,出水磷酸根质量浓度稳定在0.3 mg·L-1以下。[结论]采用乳酸作为EBPR的进水碳源是可行的。[Objectives]Objective of the present study was to explore the feasibility of enhanced biological phosphorus removal（ EBPR） by using lactic acid as the sole carbon source. [Methods]Acclimation of EBPR sludge was carried out in an anaerobicaerobic sequencing batch reactor（ SBR） by using lactic acid as influent carbon source（ SBR-2） and compared with the control SBR,which received acetic acid as influent carbon source（ SBR-1）. Once steady state operation was achieved in SBR-2,a cyclic study for both reactors were initiated through periodic sampling and analysis of such parameters as chemical oxygen demand（ COD）,phosphate,ammonia and nitrate in the mixed liquor. EBPR sludge＇ s ability in phosphorus removal was compared between two SBRs through investigating the effects of influent P concentration on phosphate removal efficiency. [Results]In the present study,enhanced biological phosphorus removal was successfully established in both lactic acid-feed SBR-2 and acetic acid-feed SBR-1 after an acclimation period of 8 d and 31 d,respectively. Similar to acetic acid-feed EBPR sludge,lactic acid-feed EBPR sludge exhibited characteristic metabolic behavior typical to polyphosphate-accumulating organisms（ PAOs）. As compared to acetic acid-feed EBPR sludge,lactic acid-feed EBPR sludge was characterized by significantly lower values of such parameters as organic acid uptake rate,P-release rate and P-uptake rate. However this did not lead to any difference in the time period required for complete aerobic P-uptake between these two sludges and this could be attributed to significantly lower P-release observed in the lactic acid-feed SBR-2. When influent phosphate concentration was not more than 12 mg·L^-1,nearly complete phosphorus removal could be achieved for the lactic acid-feed SBR-2 as indicated by low levels of effluent phosphate concentration below 0.3 mg·L^-1. [Conclusion]EBPR could be achieved by using lactic acid as the sole carbon source.

关 键 词：聚磷菌 强化生物除磷 乳酸 碳源 

分 类 号：X505[环境科学与工程—环境工程]