咪唑类离子液体在纤维素酶降解纤维素体系中的作用  

Function of imidazolium-based ionic liquids in system of enzymatic degradation of cellulose

作　　者：范琳[1] 王少君[1] 李坤兰[1] 

机构地区：[1]大连工业大学轻工与化学工程学院,辽宁大连116034

出　　处：《化工学报》2015年第1期121-125,共5页CIESC Journal

基　　金：国家自然科学基金项目(21106011)~~

摘　　要：以1-乙基-3-甲基咪唑磷酸二乙酯([Emim]DEP)、1-乙基-3-甲基咪唑醋酸([Emim]Ac)为研究对象,考察两种离子液体(IL)对酶法降解纤维素过程的影响。采用超声辅助离子液体溶解纤维素,[Emim]DEP和[Emim]Ac分别使纤维素的结晶度降为53.6%和62.3%。在纤维素酶降解再生纤维素的过程中,当IL的加入量小于2.0%时,对酶解过程起促进作用,其中加入量为0.5%时,促进作用最强,酶解率分别提高了11.4%和17.5%。在最优条件下(加入0.5%IL),测量纤维素酶系中各个酶的酶活,结果表明离子液体对β-葡萄糖苷酶起到了明显的促进作用,分别使该酶酶活提高了120%和87%。离子液体降低了纤维二糖对葡聚糖外切酶的抑制作用,提高了酶解效率。Taking 1-ethyl-3-methylimidazolium diethyl phosphate （[Emim]DEP） and 1-ethyl-3-methylimidazolium acetate （[Emim]Ac） as research targets, the influence of the two ionic liquids （IL） on the process of enzymatic degradation of cellulose was investigated. By using ultrasound to assist ionic liquids in dissolving cellulose, [Emim]DEP and [Emim]Ac made the crystallinity of cellulose decrease to 53.6%and 62.3%respectively. In the process of enzymatic degradation of regenerated cellulose, when the dosage of IL was less than 2.0%, IL acted as promoter, and when the dosage of IL was 0.5%, the promoting effect of IL was the strongest. Enzymatic hydrolysis rate increased by 11.4% and 17.5% respectively. Under the optimal conditions （add in 0.5% IL）, the enzyme activities of every enzyme in the cellulose system were measured. Ionic liquids had an obvious promoting effect on β-glucosidase, increasing enzyme activity by 120% and 87% respectively. Ionic liquids reduced inhibition of exoglucanase by cellubiose, and improved efficiency of enzymatic degradation of cellulose.

关 键 词：离子液体 纤维素 纤维素酶 溶解 降解 

分 类 号：TQ352.2[化学工程]