异常黑胆质成熟剂对抑郁症模型大鼠海马细胞外信号调控激酶信号转导通路的影响  

Effects of Abnormal Savda Munziq on hippocampal extracellular signal-regulated kinase signal transduction pathway of depression model rats

作　　者：哈力旦木.吾加不都 古孜力努尔.依马木 阿不都热衣木.肉孜 吐尔逊怕夏.吾布力卡斯木 阿不都热依木.玉苏甫 

机构地区：[1]新疆医科大学维吾尔医学院,乌鲁木齐830011 [2]新疆维吾尔自治区维吾尔医药研究所,乌鲁木齐830011

出　　处：《新疆医科大学学报》2015年第1期26-29,共4页Journal of Xinjiang Medical University

基　　金：国家自然科学基金(81360667)

摘　　要：目的探讨异常黑胆质成熟剂对抑郁症模型大鼠大脑细胞外信号调控激酶信号转导通路(ERK)的影响。方法选取Sprague-Dawley雄性大鼠84只,随机分为6组:正常对照组、阳性对照组、抑郁症模型组及异常黑胆质成熟剂低、中、高剂量组(ASMq低、中、高分别为1.5、3.0、6.0g·kg-1·d-1,连续给药4w),每组14只。除正常对照组外,其余各组均采用轻度不可预见性慢性应激刺激(Chronic mild unp redictable stress,CMUS)建立抑郁症大鼠模型,通过体质量测量、糖水摄取量综合评价大鼠行为学改变,在对ERK信号转导通路中Ras和RSK蛋白表达水平测量的基础上,采用重复测量和单因素方差分析方法,揭示异常黑胆质成熟剂的抗抑郁作用。结果与正常对照组比较,抑郁症模型组体质量增幅明显降低(P<0.05),糖水摄入量显著降低(P<0.05),差异均有统计学意义。与正常对照组比较,抑郁症模型组大鼠海马Ras和RSK蛋白表达显著降低(P<0.05),与抑郁症模型组比较,异常黑胆质成熟剂低、中、高剂量组与阳性对照组海马Ras和RSK蛋白表达显著升高(P<0.05),差异均有统计学意义。结论异常黑胆质成熟剂可能通过上调Ras/RSK/ERK信号转导通路起到抗抑郁作用。Objective To observe the effects of abnormal savda munziq on hippocampal Extracellular signal-regulated kinase signal transduction pathway of Depression model rats.Methods 84 male SD rats were randomly divided into normal control group,the positive control group,abnormal savda munziq low,me-dium and high dose groups （respectively 1.5,3.0,6.0 g·kg-1·d-1）,depression model group,roughly 14 in each group.The normal control group excluded,unpredictable mild chronic stressors （Chronic mild unpredictable stress,CMUS）was used to determine rat model of depression in the other groups.The body mass,sugar intake were measured to evaluate behavioral changes in rats.On the basis of the ERK signal transduction pathway RAS/ RSK protein expression, RM （Repeated Measurements ） and ANOVA （Single Factor Analysis of Variance method）were used to explore the antidepressant effect of abnormal savda Munziq.Results Compared with the control group,the depression model group had a significantly- decreased weight gain and sucrose intake （P 〈0.05）;Compared with normal control group,depression model group showed a significant reduction in hippocampal and RSK Ras protein expression （P 〈0.05）;Compared with those in depression model group,hippocampal and RSK Ras protein expression increased significantly in the abnormal savda Munziq low,medium,high dose groups and positive control group, having statistical significance （P〉0.05）.Conclusion Abnormal Savda Munziq （ASMq）may play an anti-depressant role by improving Ras/RSK/ERK Signal Transduction Pathway.

关 键 词：异常黑胆质成熟剂 抑郁症 

分 类 号：R749.92[医药卫生—神经病学与精神病学] R29[医药卫生—临床医学]