新疆维吾尔族HPV16感染的中晚期宫颈癌妇女HLA－DRB1及HLA-DQB1基因多态性和细胞因子相关性研究  

The correlation between Human leukocyte antigen and cytokines in HPV16 infected Xinj iang Uyghur women in advanced cervical cancer

作　　者：李亚伟[1] 古丽娜·库尔班[1] 谭遥[1] 王若峥[1,2] 

机构地区：[1]新疆医科大学附属肿瘤医院放疗中心 [2]新疆医科大学新疆肿瘤学重点实验室,乌鲁木齐830011

出　　处：《新疆医科大学学报》2015年第1期30-34,共5页Journal of Xinjiang Medical University

基　　金：科技部国际合作项目（2012DFA31560）

摘　　要：目的：探讨新疆维吾尔族妇女 HPV16感染的中晚期宫颈癌 HLA-DRB1和 HLA-DQB1的等位基因分布及其与 IFN-γ、IL-2、IL-6、IL-10的关联性。方法采集经新疆医科大学附属肿瘤医院病理科明确诊断为宫颈癌Ⅱb～Ⅳb期39例患者治疗前的血标本（宫颈癌组），同时采集居住在新疆地区健康女性50例血标本（健康对照组）。测定 HLA-DRB1和 HLA-DQB1基因亚组分型，并检测细胞因子 IFN-γ、IL-2、IL-6、IL-10的水平。对比不同临床分期患者与健康对照组细胞因子的差异；根据患者 HLA-DRB1和 HLA-DQB1基因亚组分组，比较不同基因亚组间细胞因子水平的差异。结果检出 HLA-DRB1等位基因8个，检出 HLA-DQB1等位基因4个，其中HLA-DRB1＊07、HLA-DQB1＊02、HLA-DQB1＊03在新疆维吾尔族 HPV16感染的中晚期宫颈癌组的构成比分别为44．74％、56．41％、35．90％；健康对照组与维吾尔族 HPV16感染Ⅱb和Ⅲb期宫颈癌组 IFN-γ、IL-2、IL-6、IL-10水平差异均有统计学意义（P ＜0．05）；单因素方差分析显示各 HLA-DRB1、HLA-DQB1等位基因之间 IFN-γ、IL-2、IL-6、IL-10水平差异均无统计学意义。结论 HLA-DRB1＊07、HLA-DQB1＊02、HLA-DQB1＊03为新疆维吾尔族 HPV16感染的中晚期宫颈癌优势表达基因；IFN-γ、IL-2、IL-6、IL-10与宫颈癌的发生、发展可能存在关联性。Objective To analyze the distribution of HLA-DRB1 and-DQB1alleles in HPV16 infected Xin-jiang Uygur women in advanced cervical carcinoma,and to investigate the correlation between HLA-DRB1,-DQB1 and IFN-γ,interleukin-2 （IL-2）,interleukin-6 （IL-6）,interleukin-10 （IL-10）in patients with cervical cancer.Methods We collected 3 9 blood samples of cervical carcinoma patients diagnosed by pathology as cervical cancer in stage Ⅱb-Ⅳb before the treatment in our hospital,and we gathered 50 blood samples of healthy women living in Xingjiang.We determined the genetic subtypes of HLA-DRB1 and HLA-DQB1,and we measured the concentration of IFN-γ,interleukin-2 （IL-2）,interleukin-6 （IL-6）, interleukin-10 （IL-10）.We had a comparison of the difference of cytokines between patients with different clinical stages and healthy control group.According to genetic subtypes of HLA-DRB1 and HLA-DQB1, 〈br〉 we also had a comparison of the concentration of cytokine （CK）in different genetic subtypes.Results Eight HLA-DRB1 alleles and four HLA-DQB1 alleles were found,the rates of HLA-DRB1＊07,HLA-DQB1＊02 and HLA-DQB1＊03 were 44.74%,56.41% and 35.90% respectively.We had a comparison of concentration of IFN-γ, interleukin-2 （IL-2 ）, interleukin-6 （IL-6 ）, interleukin-10 （IL-10 ） between healthy control group and HPV16 infected Xinjiang Uygur patients in advanced cervical cancer with stageⅡb-Ⅲb group,there were statistical significances （P〉0.05）.Single factor analysis of variance indicated that the difference of concentration of IFN-γ,IL-2,IL-6,IL-10 showed no statistical significance.Conclu-sion HLA-DRB1＊07,HLA-DQB1＊02 and HLA-DQB1＊03 were the gene of dominant expression in HPV16 infected Xinjiang Uygur patients in advanced cervical cancer.There may be a correlation between the occurrence,development of cervical cancer and IFN-γ,IL-2,IL-6,IL-10.

关 键 词：宫颈癌 人类白细胞抗原 细胞因子 

分 类 号：R737.33[医药卫生—肿瘤]