慈菇多糖对免疫功能低下小鼠免疫调节作用  

Immnoregulation effect of Sagittaria sagittifolia polysaccharide in immunocompromised mice

作　　者：吴小南[1] 杨雪帆[1] 朱萍萍[1] 黄芳[1] 

机构地区：[1]福建医科大学公共卫生学院营养与食品安全教研室,福建福州350108

出　　处：《中国公共卫生》2015年第1期73-75,共3页Chinese Journal of Public Health

基　　金：福建省科技计划项目(2012N0014);福建省医学创新课题(2012-CXB-14)

摘　　要：目的探讨慈菇多糖对免疫功能低下小鼠免疫功能影响。方法清洁级ICR雌性小鼠50只,按体重随机分为5组:对照组,模型组,慈菇多糖高、中、低剂量组(300、200、100 mg/kg),连续灌胃慈菇多糖28 d,对照组和模型组灌胃等量蒸馏水;第21 d,对照组皮下注射等量生理盐水,其余4组小鼠皮下注射氢化可的松25 mg/kg,连续6 d(制备免疫功能低下模型),观察各组小鼠体重变化、脏器(胸腺、脾脏、肝脏)指数、巨噬细胞吞噬功能等指标。结果与对照组比较,模型组小鼠胸腺、脾和肝指数[分别为(1.255±0.319)、(2.380±0.574)、(40.442±1.882)mg/g]明显降低,巨噬细胞吞噬指数(1.794±0.514)降低(P<0.05);与模型组比较,低、中、高剂量慈菇多糖组小鼠胸腺指数[分别为(1.848±0.130)、(1.835±0.243)、(1.708±0.441)mg/g]、脾指数[分别为(3.433±0.250)、(3.386±0.577)、(2.602±0.609)mg/g]和肝指数[分别为(45.478±2.683)、(45.423±2.109)、(44.934±2.337)mg/g]均明显升高(均P<0.05),巨噬细胞吞噬指数[分别为(2.274±0.590)、(2.406±0.246)、(2.462±0.444)]升高(P<0.05)。结论慈菇多糖在一定程度上能够提高小鼠非特异性免疫力。Objective To explore the effects of Sagittaria sagittifolia polysaccharide on immunologic function in immu- nocompromised mice. Methods Fifty ICR female mice were randomly divided into five groups（normal control group,model group, high- [300 mg/kg·d], midium- [200 mg/kg ·d], and low-dose [100 mg/kg·d] polysaccharide group ） by body weight. Sagittaria sagittifolia polysaccharide was administrated intragastrically to the mice by gavage once a day for 28days. The normal control and model group were treated with distilled water. On the 21st day, the mice in ormal control group was injected with normal saline and the mice in another 4 groups were injected with hydrocortisone of 25 mg/kg for 6 days. The body weight change, organ index（ thymus, spleen and liver） and phagocytic function of macrophages of the mice were observed in the study. Results The thymus, spleen, and the liver index were 1. 255 ± 0. 319,2. 380 ± 0. 574, and 40. 442 ± 1. 882 mg/g for the mice in model group and were significantly lower than those of the normal control group （P 〈0. 05 for all）. The macrophage phagocytic index（ 1. 794 ± 0. 514）of the model group was lower than that of the normal control group（P 〈0. 05）. The thymus index was 1. 848 ±0. 130,1. 835 ±0. 243 ,and 1. 708 ±0. 441 mg/g for the mice in low-, midium- and high-dose group, and the spleen index （ 3. 433 ± 0. 250, 3. 386 ± 0. 577, and 2. 602 ± 0. 609 rag/g） and the liver index（45. 478 ±2.683,45.423 ±2. 109 ,and 44. 934 ±2. 337 mg/g） for the mice in low-, midium- and high dose polysaccharide groups were significantly higher than those of the the model group （ P 〈 0. 05 for all）. The macrophage phagocytic index （ 2. 274 ± 0. 590,2. 406 ± 0. 246, and 2.462 ± 0. 444） of the three polysaccharide groups were all higher than those of the model group. Conclusion Saginaria saginifolia polysaceharide can enhance nonspecific immunity of mice in a certain extent.

关 键 词：慈菇多糖 免疫调节 脏器指数 巨噬细胞吞噬指数 

分 类 号：R994.4[医药卫生—毒理学]