小鼠肺癌原位模型的建立  

Establishment of orthotopic lung cancer model in mice

作　　者：胥孜杭 刘菲[1] 邹纯朴[1] 朱诗国[1] 陈晓[1] 

机构地区：[1]上海中医药大学基础医学院,上海201203

出　　处：《中国医药导报》2015年第1期15-18,F0003,共5页China Medical Herald

基　　金：上海中医药大学一流学科科研创新基金项目(编号A2-C130506);上海中医药大学研究生"创新能力培养"专项科研项目(编号A2-P3550801)

摘　　要：目的肺癌的发病率和病死率居高不下,而目前用于实验研究的肺癌动物模型存在较大的局限性,故本研究认为建立一个稳定、类似于人体肿瘤微环境的肺癌原位模型是深入研究肺癌的基础。方法直接将稳定表达虫萤光素酶的鼠Lewis肺腺癌细胞与基质胶的混悬液注射于C57 BL/6小鼠左肺,无需打开胸腔只需将小鼠左胸部皮肤剪开约1 cm小口,定期利用小动物活体成像系统中的生物发光技术监测小鼠肿瘤形成及转移情况,并将小鼠处死后立即进行肺部组织活体取材,采用石蜡包埋、切片、HE染色做出病理学诊断。结果采用肺内注射的原位造模法成瘤率高,Lewis肺腺癌细胞数量只需0.5×105个即可成瘤,成功率达90%以上,且利用活体成像系统中的生物发光技术能监测到模型小鼠肿瘤可转移至对侧胸廓、对侧肺组织、双侧腋下及腹股沟淋巴结等。结论注射虫萤光素酶稳定表达的鼠Lewis肺腺癌细胞与基质胶混悬液构建的肺癌原位模型所需细胞数量少,成瘤率高,无手术死亡风险,转移性好,监测方便,操作简单且可重复性高,为深入研究肺癌提供了良好模型。Objective Morbidity and mortality remain high in lung cancer, but the animal lung cancer models used for the experimental research are limited, so to establish an orthotopic lung cancer model, which is stable and similar to the human tumor microenvironment, is a foundation for the further study of lung cancer research. Methods Luciferase-expressed LLC cells and matrigel matrix were directly injected into the left lung of C57BL/6 mice after cutting open a incision of the skin, which about 1 cm, and there was no need to open the chest. Small animal in vivo bioluminescence imaging system was used to monitor tumor growth and metastasis. At different time points, mice were sacrificed and the lung tissues were isolated and imbedded with paraffin. Pathological diagnosis was made by sliced and HE stained. Re-sults LLC cells （0.5＆#237;105） were enough to form an orthotopic pulmonary tumor, and the success rate was above 90%. The metastases to contralateral thoracic, contralateral lung, bilateral axillary and inguinal lymph nodes were monitored by bioluminescence technique. Conclusion The orthotopic tumor models of lung cancer can successfully built by injec-tion of luciferase-expressed LLC cells and matrigel matrix mixture. Furthermore, a few LLC cells are needed, and the tumor formation rate is high, besides there is no operation death risk. It has good metastases and high repeatability, in addition it＇s easy to operate and easy to be monitored, which provides a good method for the establishment of mouse models for further study of lung cancer.

关 键 词：肺癌 原位模型 活体监测 

分 类 号：R734.2[医药卫生—肿瘤]