川桑的种子萌发及染色体核型鉴定  

Seed Germination of Morus notabilis Schneid and Identification of Its Karyotype

作　　者：轩亚辉 李杨[1] 王圣[1] 向仲怀[1] 何宁佳[1] 

机构地区：[1]家蚕基因组生物学国家重点实验室,西南大学,重庆400715

出　　处：《蚕业科学》2014年第6期957-960,共4页ACTA SERICOLOGICA SINICA

基　　金：国家高技术研究发展计划“863”项目(No.2013AA10060-5);商务部市场运行司茧丝绸产业公共服务体系建设项目(No.3)

摘　　要：野生桑树种质资源川桑(Morus notabilis Schneid)作为桑树基因组测序的桑种,是研究桑树染色体和功能基因的重要材料。将从四川省雅安市采集的野生川桑种子人工去皮后在MS培养基上进行培养,实现了野生川桑种子室内培养条件下的萌发,种子萌发率高达85.7%。分别以萌发幼苗的叶片和根尖为材料,采用去壁低渗法制备染色体标本进行核型鉴定,结果显示川桑幼苗组织体细胞有14条染色体,并组成7对,即2n=2x=14。川桑种子的室内萌发解决了进行川桑功能基因组研究时受材料限制的难题;染色体核型鉴定的结果验证了桑树染色体基数为7(x=7)的推论。As DNA resources of mulberry genome sequencing,wild mulberry germplasm resource Morus notabilis Schneid is an important material for studies on mulberry chromosomes and functional genes. In present study,M. notabilis seeds collected from Ya＇an City,Sichuan Province,were cultured on MS medium after manually peeling the seed coats. The germination rate of M. notabilis seeds was up to 85. 7% under laboratory culture condition. The leaves and root tips from the germinated seedlings were used as material to prepare chromosome samples using wall removal and hypotonic treatment for karyotypic analysis. The result showed that the chromosome number of somatic cells of M. notabilis seedling was 14 and these 14 chromosomes were grouped into 7 pairs（ 2n = 2x = 14）. Indoor germination of M. notabilis seeds have resolved the difficulty that the studies of mulberry genomic function was restricted by unavailability of M. notabilis as experimental materials. In addition,the data provided in this research validate our proposal again that the cardinal chromosome number of mulberry species is 7（ x = 7）.

关 键 词：川桑 种子萌发 种皮剥除 染色体核型 

分 类 号：S888.3[农业科学—特种经济动物饲养] S330.39[农业科学—畜牧兽医]