细胞因子对肺癌H460细胞MMP表达的影响及地塞米松的干预作用  

Effect of dexamethasone on cytokine to infuence MMP expression of H460 cells

作　　者：卢春玲[1] 闫圣杰[1] 常双喜[1] 

机构地区：[1]郑州市第一人民医院呼吸内科,450000

出　　处：《国际呼吸杂志》2014年第24期1851-1854,共4页International Journal of Respiration

摘　　要：目的 在肺癌H460细胞的三维立体培养过程中,直接观察细胞因子[肿瘤坏死因子α（TNF-α）和IL-1β]对肺癌H460细胞关于基质金属蛋白酶-2（MMP-2）和MMP-9表达的诱导作用及地塞米松的干预作用.方法 在肺癌H460细胞三维立体培养过程中加入细胞因子TNF-α和IL-1β来诱导MMP的表达,同时加入地塞米松干预MMP-2和MMP-9的表达;用明胶酶谱法测定MMP-2、MMP-9,同时在培养5d后测定胶原含量大小.结果 TNF-α和IL-1β诱导培养在立体胶原内的肺癌H460细胞产生大量MMP-2和MMP-9,并促进其活化,引起大量胶原降解,地塞米松抑制了MMP-2及MMP-9的表达及活化,进而抑制了胶原的降解,对抗了细胞因子TNF-α和IL-1β对胶原的降解作用.结论 细胞因子TNF-α和IL-1β可诱导MMP的表达,MMP可直接降解肺内胶原,进而促进肺癌的侵袭或转移;地塞米松通过抑制MMP的表达与活化对抗MMP对肺组织的破坏作用,进而抑制了肺癌的侵袭与转移.Objective To investigate the effects of dexamethasone on cytokines [tumor necrosis factor-α （TNF-α） and interleukin-1β （IL-1β）] to induce expressions of matrix metalloproteinase-2 （MMP-2） and MMP-9 in lung cancer H460 cells.Methods Human lung cancer H460 cells were cultured in three-dimensional collagen gels.TNF-α and IL-1β were added to induce expressions of MMP-2 and MMP-9 in lung cancer H460 cells,and dexamethasone was added to the culture media at the same time.The expressions of MMP-2 and MMP-9 were detected by gelatin zymography.Collagen content was determined on the 5th day.Results TNF-α and IL-1β induced lung cancer H460 cells to produce MMP-2 and MMP-9 in latent forms and activated MMP,resulting in generously collagen degradation.But dexamethasone inhibited the effect of cytokines （TNF-a and IL-1β） to induce productions of MMP-2 and MMP-9.Conclusions TNF-α and IL-1β may induce production and activation of MMP-2 and MMP-9,and MMP may be involved in the lung tissue destruction in the invasion and metastasis of lung cancer,however,dexamethasone may inhibit the effect of cytokine in the lung tissue destruction in the invasion and metastasis of lung cancer.

关 键 词：地塞米松 基质金属蛋白酶 三维立体培养 细胞因子 肿瘤坏死因子α 白介素1Β 肺癌 

分 类 号：R734.2[医药卫生—肿瘤]