人正常宫颈脱落细胞及宫颈癌细胞的AFM观察研究  

Study of human normal cervical cell and cervical cancer cells by AFM

作　　者：高洪菲 尚秀敏 宋雨霖 孙滨州 韩毅敏[1] 

机构地区：[1]哈尔滨医科大学附属肿瘤医院妇科,哈尔滨150040 [2]黑龙江省牡丹江市妇女儿童医院,牡丹江157000

出　　处：《现代妇产科进展》2014年第11期853-855,共3页Progress in Obstetrics and Gynecology

基　　金：黑龙江省留学归国科学基金项目(No:LC2011C31);哈尔滨市科技局课题(No:2011RFLYS022)

摘　　要：目的:探讨原子力显微镜(AFM)在人宫颈脱落细胞及宫颈癌细胞微观形貌表征方面的应用。方法:利用液基薄层细胞学检测技术制备细胞样品,应用原子力显微镜分别观察人宫颈脱落细胞及宫颈癌细胞的微观形态。结果:癌变宫颈细胞核独立分布,尺寸变大,且易于聚集,细胞质分散在细胞核周围;正常宫颈细胞的细胞核被纳米条状的细胞质覆盖,成为一个整体。结论:采用AFM检测液基薄层细胞学检测技术制备的细胞样品的微观形貌能反映出与宏观光学显微镜检测获得相同的结论,是一种简易的检测方法。癌变宫颈细胞和正常宫颈细胞在微观上存在明显不同:癌变宫颈细胞核的尺寸增大、聚集在一起;而正常宫颈细胞被纳米条状的细胞质所覆盖。Objective：To explore applications of AFM （Atomic Force Microscope） in human normal cervical cell and cervical cancer cells morphology characterization. Methods：To utilize the liquid based cytology detection technology to prepare cell samples. AFM was em-ployed to image the cervical normal and cancer cells. Results：Canceration of Cervical cell nu-cleus was independent distribution with the properties of large size and easy to assemble. Cyto-plasm disperses in the perinuclear. The normal cervical cell nuclear is covered by nano strips of cells,forming a whole body. Conclusion：AFM and the liquid based cytology detection technolo-gy preparing cell samples are used to study microcosmic morphology of cells. The obtained con-clusions are comparable of those achieved by the macroscopic morphology characterization de-tection technology. AFM is verified to be an easy detection method. Differences between cancer-ation of Cervical cells and normal cervical cell are obvious in microcosmic morphologies. Can-ceration of Cervical cell nucleus becomes large in size and is easy to assemble. Normal cervical cell is covered by nano strips of cells.

关 键 词：原子力显微镜(AFM) 人宫颈癌细胞 微观形貌 

分 类 号：R737.31[医药卫生—肿瘤]