大鼠肝卵圆细胞系WB-F344恶性转化模型的构建及NOD/SCID小鼠成瘤实验  

Induced transformation of rat oval cells line WB-F344 and tumor formation in NOD / SCID mice

作　　者：舒曼[1] 张明辉[1] 何潇潇[1] 丁力[1] 林原[1] 曹清华[1] 杨峥[1] 薛玲[1] 

机构地区：[1]中山大学附属第一医院病理科,广州510080

出　　处：《临床与实验病理学杂志》2014年第12期1354-1357,1364,共5页Chinese Journal of Clinical and Experimental Pathology

基　　金：国家自然科学基金(81172342);广东省自然科学基金(9151008901000117;S2011010004675)

摘　　要：目的通过N-甲基-N’-硝基-N-亚硝基胍(MNNG)处理大鼠肝卵圆细胞系WB-F344,使其发生恶性转化,为研究肝癌的发生、发展提供有用的体外模型。方法以MNNG作用于WB-F344细胞系,使之发生恶性转化,并从形态学、遗传学、软琼脂克隆形成、NOD/SCID小鼠成瘤等方面加以验证。结果经MNNG诱导数代的WB-F344细胞出现恶性特征,表现为细胞形态改变,异倍体核型增多,肿瘤标志物表达增高,非锚定克隆能力增强,接种于NOD/SCID小鼠皮下可成瘤。结论肝卵圆细胞系WB-F344在MNNG的作用下可成为肝癌发生的有效体外模型。Purpose To study the malignant transformation after treating rat oval cell line （ WB-F344 ） with chemical carcinogen N-methyl-N′-nitro-N-nitrosoguanidine （ MNNG） . Methods WB-F344 cells were cultured with MNNG for severe times. The biological characteristics of induced cells were detected through the following methods：to check proliferation activity by flow cytometry analysis, to examine malignant transformation degree of induced cells by soft agar assay and tumor formation in NOD/SCID mice, and to investi-gate the transcriptional and protein levels of hepatocellular carcinoma marker GGT, GST-P by real time-PCR. Results Oval cells in-duced by MNNG showed changes in biological characteristics and malignant molecular markers. Conclusion Hepatic oval cells model is successfully established, which can be confirmed by tumor formation in NOD/SCID mice.

关 键 词：肝卵圆细胞 恶性转化 成瘤实验 NOD/SCID小鼠 

分 类 号：R73-3[医药卫生—肿瘤]