糖尿病大鼠肾脏组织中XBP-1s和ADRP的表达  

Expression of spliced XBP-1s and ADRP in kidney of diabetic rats

作　　者：朱琳[1] 郝军[2] 赵松[2] 赵雪[2] 陈宁[2] 郑书深[2] 段惠军[2] 

机构地区：[1]河北医科大学第三医院肌电图室,石家庄050051 [2]河北医科大学基础医学院病理学教研室,石家庄050017

出　　处：《临床与实验病理学杂志》2014年第12期1371-1374,共4页Chinese Journal of Clinical and Experimental Pathology

基　　金：河北省高等学校科学技术研究优秀青年基金(Y2011108);河北省科技计划项目(13277740D);河北省高等学校科学技术项目(ZD2014103)

摘　　要：目的探索糖尿病大鼠肾脏拼接型X盒子结合蛋白1(spliced X-box binding protein-1,XBP-1s)和脂肪分化相关蛋白(adipose differentiation related protein,ADRP)在糖尿病大鼠肾脏组织中的表达及二者表达的相关性。方法腹腔注射STZ成功构建糖尿病大鼠模型,正常喂养2个月后,采用免疫组化和Western blot技术检测大鼠肾脏组织中XBP-1s和ADRP的表达。结果 XBP-1s和ADRP表达均定位于肾小管上皮细胞,对照大鼠相比,糖尿病大鼠肾小管上皮细胞中XBP-1s和ADRP表达均显著增高,分别升高2.017倍和1.544倍,差异有统计学意义(t=2.648、2.741,P<0.05)。进一步行相关性分析显示:大鼠肾脏组织中XBP-1s表达增强往往伴随着ADRP的表达增强(相关系数为0.723,P<0.05)。结论 XBP-1s表达升高可能是导致糖尿病大鼠肾脏组织中ADRP上调的因素之一。Purpose To investigate the expression of XBP-1s and ADRP in kidney of diabetic rats. Methods Diabetic rat models were successfully established by intraperitoneal injection of STZ. After two months rats were sacrificed and XBP-1s and ADRP were de-tected by immunohistochemistry and Western blot. Results XBP-1s and ADRP were located in renal tubular cells and increased by a-bout 2. 017 times and 1. 544 times in comparison with normal control rats （P〈0. 05）. Moreover, it was shown that high expression of XBP-1s was commonly accompanied with increased ADRP by Pearson correlation analysis and the correlation coefficient was 0. 723 （P〈0. 05）. Conclusion The increased XBP-1s may cause the up-regulation of ADRP in the kidney of diabetic rats.

关 键 词：糖尿病 肾小管上皮细胞 大鼠 拼接型X盒子结合蛋白1 脂肪分化相关蛋白 

分 类 号：R322.61[医药卫生—人体解剖和组织胚胎学]