SLC24A5,SLC39A9基因在黑线仓鼠及其A：CHA品系表达水平的比较分析  

Comparison of Expression Level of SLC24A5, SLC39A9 Genes in Cricetulus griseus and the Albino mutant

作　　者：刘慧芳[1,2] 孙书芳 曾林[2] 曹玉华[2] 严翔[2] 范君文[2] 孙兆增[2] 王新[1] 

机构地区：[1]青岛农业大学,青岛266109 [2]军事医学科学院实验动物中心,北京100071 [3]山东省青岛市中心血站,青岛266071

出　　处：《实验动物与比较医学》2014年第6期438-442,共5页Laboratory Animal and Comparative Medicine

基　　金：国家自然科学基金项目（31272387）

摘　　要：目的 比较可溶性物质运输蛋白SLC24A5（solute carrier family 24,member5,SLC24A5）和SLC39A9（solute carrier family 39,member9,SLC39A9）在黑线仓鼠及其白化突变系（A：CHA）皮肤组织的基因表达差异,分析这两种基因对白化性状产生的影响.方法 提取黑线仓鼠和A：CHA的皮肤组织总RNA,利用普通PCR的方法筛选引物,利用实时荧光定量PCR技术,比较分析黑线仓鼠和A：CHA皮肤组织SLC24A5、SLC39A9表达量的异同.结果 黑线仓鼠皮肤中的SLC24A5、SLC39A9基因mRNA的相对表达量分别是A：CHA组织中的2倍和3.8倍.结论 SLC24A5、SLC39A9基因在A：CHA皮肤组织的表达量下调,说明SLC24A5和SLC39A9基因与A：CHA白化性状的产生存在一定的关联性.Objective To investigate the expression level of the soluble material transport protein SLC24A5 （solute carrier family 24,member 5,SLC24A5） and SLC39A9 （solute carrier family 39,member 9,SLC39A9） between the Cricetulus griseus and its albino mutation strain,A：CHA,and analyze the function on the albino phenotype.Methods The total mRNA of skin tissue in the Cricetulus griseus and A：CHA was extacted.The PCR method was used to select primers.The expression levels of SLC24A5,SLC39A9 was analyzed by real time PCR.Results The ratio of expression level of SLC24A5,SLC39A9 between Cricetulus griseus and A：CHA were 2 and 3.8 times.Conclusion The gene expression level of SLC24A5,SLC39A9 in the A：CHA were lower than that in the Cricetulus griseus.The study strongly supports the notion that the two genes may be contribute to the albino phenotype.

关 键 词：黑线仓鼠 A: CHA SLC24A5 SLC39A9 实时荧光定量PCR 

分 类 号：Q95-33[生物学—动物学]