A Novel Chitosan-based Sponge Coated with Self-assembled Thrombin/Tannic Acid Multilayer Films as a Hemostatic Dressing  

作　　者：Xiao-fei Huang Jing-wei Jia 王征科 胡巧玲 

机构地区：[1]MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University

出　　处：《Chinese Journal of Polymer Science》2015年第2期284-290,共7页高分子科学（英文版）

基　　金：financially supported by the National Natural Science Foundation of China(Nos.21104067,21274127 and 21374099 and 51473144);Key Basic Research Development Plan(973 Program)of China(Nos.2009CB930104 and 2011CB606203);Fundamental Research Funds for the Central Universities(No.2013QNA4048);Technology Foundation for Selected Overseas Scholar of Zhejiang Province

摘　　要：In order to prepare a novel hemostatic dressing for uncontrolled hemorrhage, a porous chitosan sponge was coated with self-assembled（thrombin/tannic acid）n films, which were based on hydrogen bonding interactions between thrombin and tannic acid at physiologic p H. According to the whole blood clotting test, the coated chitosan sponges showed a significantly high rate of blood clotting due to the addition of thrombin. On the other hand, the storable half-life of immobilized thrombin is extended to 66.9 days at room temperature, which is 8.5 times longer than unfixed thrombin. It is because of the immobilization effect of, not only the porous structure of chitosan sponge but also the interactions between thrombin and tannic acid. In addition, the tannic acid has similar antibacterial effect to chitosan. Therefore, it is an excellent combination of chitosan, thrombin and tannic acid. Besides, all of materials in this research have been approved by the United States Food and Drug Administration（FDA）. So the chitosan-based sponge is a promising candidate dressing for uncontrolled hemorrhage due to its storable, bio-safe and highly effective hemostatic properties.In order to prepare a novel hemostatic dressing for uncontrolled hemorrhage, a porous chitosan sponge was coated with self-assembled（thrombin/tannic acid）n films, which were based on hydrogen bonding interactions between thrombin and tannic acid at physiologic p H. According to the whole blood clotting test, the coated chitosan sponges showed a significantly high rate of blood clotting due to the addition of thrombin. On the other hand, the storable half-life of immobilized thrombin is extended to 66.9 days at room temperature, which is 8.5 times longer than unfixed thrombin. It is because of the immobilization effect of, not only the porous structure of chitosan sponge but also the interactions between thrombin and tannic acid. In addition, the tannic acid has similar antibacterial effect to chitosan. Therefore, it is an excellent combination of chitosan, thrombin and tannic acid. Besides, all of materials in this research have been approved by the United States Food and Drug Administration（FDA）. So the chitosan-based sponge is a promising candidate dressing for uncontrolled hemorrhage due to its storable, bio-safe and highly effective hemostatic properties.

关 键 词：CHITOSAN THROMBIN Tannic acid Layer-by-Layer assembly Hemostatic dressing 

分 类 号：R318.08[医药卫生—生物医学工程] TQ314.1[医药卫生—基础医学]