Membrane protein reconstitution for functional and structural studies  

作　　者：WANG LiGuo TONGGU LiGe 

机构地区：[1]Department of Biological Structure,University of Washington

出　　处：《Science China(Life Sciences)》2015年第1期66-74,共9页中国科学（生命科学英文版）

基　　金：supported by US National Institutes of Health(R01GM096458)to Wang LiGuo

摘　　要：Membrane proteins are involved in various critical biological processes,and studying membrane proteins represents a major challenge in protein biochemistry.As shown by both structural and functional studies,the membrane environment plays an essential role for membrane proteins.In vitro studies are reliant on the successful reconstitution of membrane proteins.This review describes the interaction between detergents and lipids that aids the understanding of the reconstitution processes.Then the techniques of detergent removal and a few useful techniques to refine the formed proteoliposomes are reviewed.Finally the applications of reconstitution techniques to study membrane proteins involved in Ca2+ signaling are summarized.Membrane proteins are involved in various critical biological processes,and studying membrane proteins represents a major challenge in protein biochemistry.As shown by both structural and functional studies,the membrane environment plays an essential role for membrane proteins.In vitro studies are reliant on the successful reconstitution of membrane proteins.This review describes the interaction between detergents and lipids that aids the understanding of the reconstitution processes.Then the techniques of detergent removal and a few useful techniques to refine the formed proteoliposomes are reviewed.Finally the applications of reconstitution techniques to study membrane proteins involved in Ca^2＋ signaling are summarized.

关 键 词：membrane protein RECONSTITUTION Ca2＋ signaling Ca2＋ mediators Ca2＋-regulated enzymes Ca2＋ transducers 

分 类 号：Q73[生物学—分子生物学]