耐药性癫痫患者脑内Nurr1基因及蛋白产物的表达  

The study of mRNA and protein level expression of Nurr1 in refractory epilepsy

作　　者：李应宏[1] 莫烨[1] 李劲梅[2] 阙玉梅 张玲[1] 

机构地区：[1]攀枝花市攀钢集团总医院,四川攀枝花617023 [2]四川大学华西医院神经内科,四川成都610041

出　　处：《西部医学》2015年第1期5-8,12,共5页Medical Journal of West China

基　　金：国家自然科学基金(81071047)

摘　　要：目的研究耐药性癫痫患者颞叶和海马组织中Nurr1基因及其蛋白产物的表达,探讨其在耐药性癫痫形成中的作用。方法用RT-PCR和免疫组化联合检测40例耐药性癫痫患者(实验组)脑组织中Nurr1基因及蛋白产物的表达,并与11例对照组比较。结果 RT-PCR结果显示Nurr1mRNA水平在耐药性癫痫患者脑组织中表达较对照组增高,Nurr1/GAPDH(内参基因)灰度值在耐药性癫痫患者脑组织中为6.18,对照组为2.39(P<0.05)。免疫组化与RT-PCR结果一致,显示耐药性癫痫颞叶和海马Nurr1蛋白表达水平高于对照组(P<0.05)。结论 Nurr1基因及其蛋白产物在耐药性癫痫患者脑内表达明显增加,提示其可能参与了耐药性癫痫的发生与发展。Objective To learn Nurrl mRNA and protein expressional levels in temporal lobe and hippocampus tissue of patients with refractory epilepsy and the role it plays in mossy sprouting. Methods FQ- rtPCR, and immunohisto- chemistry were used to check Nurrl mRNA and protein levels in refractory epilepsy, comparing to the 11 cases from control group. Results Rt-PCR result showed Nurrl mRNA level was up regulated in refractory epilepsy group. The gray scale ratio of Nurrl and GADPH is 6.18 in refractory epilepsy and 2.39 in the control group （P〈0. 05）. Immunohisto- chemistry showed the protein level of Nurrl up-regulated obviously in refractory in epileptic temporal lobe, the optical density value is 0. 2417±0. 0426 in refractory epilepsy and 0. 065±0. 0524 in the control group（P〈0.05）, the similar result also got in hippocampus tissue. Conclusion The function of Nurrl is associated with mossy fiber sprouting closely, both Nurrl mRNA and protein level up regulated in refractory epilepsy indicated it play an important role in mossy fiber sprouting process.

关 键 词：NURR1基因 苔藓纤维 难治性癫痫 

分 类 号：R742.1[医药卫生—神经病学与精神病学]