CT对大鼠脂肪肝定量诊断的实验研究  

Quantitative diagnosis for fatty liver in rat through Computed Tomography

作　　者：周选民[1] 刘玥[2] 胡承雷[1] 曾蕾[2] 

机构地区：[1]湖北医药学院附属太和医院放射科,湖北十堰442000 [2]湖北医药学院附属人民医院,湖北十堰442000

出　　处：《西部医学》2015年第1期13-16,共4页Medical Journal of West China

摘　　要：目的 通过构建大鼠非酒精性脂肪肝模型,验证CT在脂肪肝定量诊断中的作用,从而发现其特点,明确优、劣势,提高诊断的特异性和灵敏性.方法 Wistar大鼠60只,适应性饲养1周后,按照随机对照原则,分为正常对照组（对照组）和非酒精性脂肪肝模型组（模型组）,每组30只.对照组一般饲料饲养,模型组给予高脂饲料.每组分别在第8、12、16周随机取10只大鼠,以1.0％戊巴比妥钠（3.0 ml/100 g体重,ip）麻醉大鼠,称取体重,肝脏行CT检查后,在无菌条件下手术开腹,取肝右叶中部一块5mg组织用10％甲醛固定,HE染色,观察组织病理变化.结果 病理表现：对照组切片示肝细胞无脂肪变性.模型组8周为单纯性脂肪肝,12周模型组为中～重度脂肪肝,16周始从脂肪性肝炎进展到脂肪性肝纤维化;16周肝组织有炎性坏死.CT示：对照组图像为肝实质密度均匀.模型组8周有明显的脂肪变,图像可见肝脏轻度增大,肝实质密度减低;12周后表现更为明显,肝脏进一步增大且出现肝缘圆钝,肝实质密度比血液还低;16周后肝实质密度继续降低.体重、脂变评分和CT评分：对照组8周分别为（515.13±40.12）、（1.00±0.00）、（62.3±7.4）;模型组为（578.78±42.25）、（2.40±0.55）、（46.8±5.3）.对照组12周分别为（592.45±49.23）、（1.00±0.00）、（61.5±6.8）;模型组分别为（643.78±53.89）、（2.65±0.43）、（33.4±3.7）.对照组16周分别为（683.34±35.69）、（1.00±0.00）、（60.9±7.5）;模型组分别为（659.51±54.87）、（2.84±0.41）、（16.2±3.9）.对照组与模型组间在不同时期体重、脂变评分和CT评分比较均有显著性差异（P＜0.05）.模型组HE染色确诊,轻度脂肪肝12例,中度10例,重度脂肪肝8例.按CT阈值标准的诊断符合率为53.3％;按血管相对密度值分级标准的诊断符合率为73.3％;按肝/脾CT值比值的诊断诊断符合率为90％.结论 CT值的高低与肝�Objective Fatty liver models in rats were constructed to verify Computed Tomography （CT） examination＇s effects in the quantitative diagnosis of fatty liver, and to discover Computed Tomography （CT） examination＇s features for fatty liver and make CT＇s diagnosis advantage and disadvantage explicit in order to improve CT＇s specificity and sensitivity for fatty liver＇s diagnosis. Methods 60 Wistar rats, according to principle of random and comparison, were divided into the normal group and the fatty liver group. The rats in the normal group were fed with regular forage, and rats in the fatty liver group were fed with high fat diet. 10 rats were taken randomly from each group after feeding for 8, 12 and 16weeks, and taken CT examination, then taken open appendectomy under aseptic conditions. 5 mg specimen from the middle part of right liver were fixed with 10% formaldehyde, and stained with l＇tematoxylin-eosin to observe his- topathological change. Results Pathology showed that there was no steatosis in the normal group; The specimen hepar adiposum appeared 8 weeks after treatment, Moderate - Severe fatty liver appeared 12 weeks after treatment, steatohep- atitis and hepatic fibrosis appeared 16 weeks after treatment. CT showed rats in the normal group had the same liver pa- renchyma density. The fatty degeneration, liver volume increased and liver parenchyma density decreased appeared 8, 12 and 16 weeks after treatment. There were significant differences in weight, score of fatty degeneration, CT value among the normal group and the fatty liver group during different period. There were 12 cases of simple hepar adiposum and 10 cases of moderate fatty liver and 8 cases of severe fatty liver diagnosed with HE stained. The correct diagnosis rate was 53.3 % depended on CT scope standard. The correct diagnosis rate was 73.3% depended vesscle density standard. The correct diagnosis rate was 90% depended on liver/spleen CT value standard. Conclusion There is negative correlation between CT and liver fat d

关 键 词：计算机断层扫描摄影术 大鼠 非酒精性脂肪肝 定量诊断 

分 类 号：R575.5[医药卫生—消化系统]