复合因素造模法建立血热出血大鼠模型及初步评价  

Preliminary evaluation on the establishment of rat models of bleeding caused by heat syndrome by using combined factors

作　　者：段红福 荣春蕾[2] 张文峰 崔瑛[4] 

机构地区：[1]郑州市中医院,郑州450008 [2]河南中医学院第一附属医院,郑州450008 [3]河南省宛西制药股份有限公司,郑州450008 [4]河南中医学院/呼吸疾病诊疗与新药研发河南省协同创新中心,郑州450008

出　　处：《中华中医药杂志》2015年第1期259-263,共5页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.30772794)~~

摘　　要：目的:建立符合中医辨证的血热出血大鼠模型,并对此模型进行初步的评价和验证。方法:采用腹腔注射内毒素和皮下注射干酵母相结合的方法建立血热出血大鼠模型,以体温差、凝血指标[凝血酶时间(TT)、凝血酶原时间(PT)、活化部分凝血酶时间(APTT)、纤维蛋白原(FIB)]、病理组织学观察为指标进行初步评价,并以清热凉血药生地黄对此模型进行验证。结果:与正常组相比,模型组体温显著升高(P<0.05),而生地黄高剂量组的体温则显著下降(P<0.05);模型组PT延长(P<0.05),FIB升高(P<0.05),TT缩短(P<0.05),生地黄低剂量组可使TT显著延长(P<0.05),使FIB显著降低(P<0.05);模型组大鼠主要脏器发生明显病理学变化:瘀血、凝血、微血栓,出血等,而生地黄对这些病理变化有明显的改善作用。结论:本研究所采用的复合因素造模法可成功建立大鼠血热出血模型,可为血热出血证、清热凉血药的实验研究奠定基础。Objective： To establish rat models of bleeding caused by heat syndrome, and evaluate and validate the rat models preliminary. Methods： Rat models of bleeding caused by heat syndrome were established by using the combination method of intraperitoneal injection of endotoxin and subcutaneous injection of dried yeast. Conducted a preliminary evaluation based on the following indicators： temperature difference, coagulation indicator （PT, TT, APTT and FIB） and pathological changes, and conducted a preliminary validation with unprocessed rehmannia root drug for clearing heat and cooling blood. Results： Compared with control group, the temperature of rats in model group increased （P〈0.05）, however, the temperature of rats in high dosage of unprocessed rehmannia root group decreased significantly （P〈0.05）. The coagulation parameters such as PT, TT, FIB of rats in model group were apparent abnormality compared with control group, for example： PT was prolonged （P〈0.05）, FIB was increased （P〈0.05）, and TT was shortened （P〈0.05）, however, unprocessed rehmannia root could extend the TT （P〈0.05） and decreased the FIB significantly （P〈0.05）. There were obvious pathological changes in main organs of rats in model group, such as blood stasis, cruor, microthrombus, bleeding and so on, however, unprocessed rehmannia root could improve the pathological changes obviously. Conclusion： Rat models of bleeding caused by heat syndrome could be established by using combined factors, and this could lay the foundation of the experiment researches of bleeding caused by heat syndrome and drugs for clearing heat and cooling blood.

关 键 词：血热出血模型 凝血指标 瘀血 凝血 出血 

分 类 号：R-332[医药卫生] R228