光照条件改变对青春期前雄性金黄地鼠性腺轴的影响  

Effects of photoperiodic alteration on gonadal axis in male golden hamsters before puberty

作　　者：薛媛媛[1] 林燕燕 俞建[1] 孙雯[1] 汪永红[1] 

机构地区：[1]复旦大学附属儿科医院中医科,上海201102 [2]厦门市妇幼保健院,厦门361004

出　　处：《中华中医药杂志》2015年第1期280-283,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金面上项目(No.81072841);国家临床重点专科(中医儿科)~~

摘　　要：目的:观察光照改变对雄性金黄地鼠下丘脑-垂体-性腺轴的影响。方法:2日龄雄性金黄地鼠40只,随机分为正常组(12h,50lux)、16h弱光组(16h,50lux)、16h强光组(16h,350lux)和20h强光组(20h,350lux),光照干预至28日龄;放免法检测血清睾酮(T)、黄体生成素(LH)、促卵泡激素(FSH)水平;Real-time PCR法检测下丘脑促性腺激素释放激素(Gn RH)、促性腺激素抑制激素(Gn IH)、褪黑素受体1c亚型(Mel1c),垂体Gn RHR、GPR147 m RNA表达变化。结果:光照组金黄地鼠出现躁动不安,皮毛光泽度差,体质量偏轻等类似阴虚火旺证候表现。与正常组比较,各光照组T水平显著上升(P<0.05);下丘脑Mel1c m RNA表达水平显著下降(P<0.01,P<0.05);16h弱光组和20h强光组下丘脑Gn IH m RNA表达水平显著升高(P<0.05)。结论:延长光照时间、增加光照强度可使雄性地鼠出现一定的阴虚火旺表现。光照时间延长、强度增加可引起雄性地鼠睾丸发育提前,其机制可能与褪黑素受体、Gn IH有关。Objective： To investigate the effects of photoperiod alteration on hypothalamic-pituitary-gonadal axis in male golden hamsters before puberty. Methods： 40 male golden hamsters of 2-day-old were randomly divided into four groups, N group （12h, 501ux）, M 1 group （16h, 501ux）, M2 group （16h, 3501ux） and M3 group （20h, 3501ux）. Artificial light was taken instead of sunlight until the pups were 28-day-old. Serum levels of testosterone （T）, follicle stimulating hormone （FSH） and luteinizing hormone （LH） were determined by using ELISA. The mRNA expression of hypothalamic GnRH, GnlH and Mellc genes were detected by using Real-time PCR while the expression of GnRH and GPR147 genes in the pituitary gland were detected. Results： The symptoms of hyperactivity of fire due to yin deficiency syndrome were showed in illumination groups, such as restless, poor in lustrousness, decreasing body weight. Compared with N group, the T levels in illumination groups were increased significantly （P〈0.05）. The mRNA expression of hypothalamic Mellc was decreased significantly （P〈0.05, P〈0.01）. The mRNA level of hypothalamic GnlH in MI group and M3 group were increased significantly （P〈0.05）. Conclusion： The syndrome of hyperactivity of fire due to yin deficiency in male hamsters was induced by extending the photoperiod and increasing high light intensity. Long photoperiod and high light intensity could induce testicular development in advance in male hamsters through regulating expression of GnlH and Mellc.

关 键 词：光照改变 促性腺激素释放激素 促性腺激素抑制激素 褪黑素受体1c亚型 阴虚火旺 

分 类 号：R-332[医药卫生]