基于石墨烯的汞离子检测生物传感器的研究  

Biosensor Based on Graphene for Detection of Hg^(2+)

作　　者：邓大庆[1,2,3] 孙姜[1,3] 王立梅[1,3] 朱颖越[1,3] 齐斌[1,3] 

机构地区：[1]常熟理工学院生物与食品工程学院,江苏常熟215500 [2]中国矿业大学化工学院,江苏徐州221000 [3]苏州食品生物技术重点实验室,江苏常熟215500

出　　处：《化学试剂》2015年第1期59-61,共3页Chemical Reagents

基　　金：江苏省基础研究计划(自然科学基金)项目(BK20130379);江苏省高校自然科学研究面上资助经费项目(13KJB550001);苏州市科技计划基金资助项目(SS201335)

摘　　要：基于氧化型石墨烯优越的荧光淬灭效率及T-Hg2+-T的特异性结合作用,设计了一种新型的汞离子生物传感器。通过整个体系荧光强度的变化实现对样品中Hg2+离子浓度的检测。设计的汞离子检测传感器具有很高的灵敏度,Hg2+离子浓度在0.1~50 nmol/L的范围内与荧光强度的绝对值呈现良好的线性关系,检测限为0.08 nmol/L。此外,在特异性实验中,该方法也具有良好的选择性和特异性。在实际样品添加实验中,测出其回收率为96%~106%。该方法简单、快速、特异性强,可以应用于农产品中Hg2+离子的快速检测。A novel biosensor was designed based on the unexceptionable fluorescence quenching efficiency of graphene oxide and T-Hg^2＋-T specific binding for detection of Hg^2＋. The concentration of Hg^2＋was detected by fluorescence intensity variation. The sensor was highly sensitive. There was a good linear relation between the concentration of Hg^2＋and the absolute value of fluorescence intensity within 0. 1 to 50 nmol / L and the detection limit was 0. 08 nmol / L. In addition,the specificity was outstanding and the recovery for water samples was in the range of 96% - 106%. This method was simple,fast,specific,and could be used to detect Hg^2＋in agricultural products.

关 键 词：汞离子 氧化型石墨烯 荧光淬灭 

分 类 号：O655[理学—分析化学]