MCF-7与MDA-MB-231细胞逃避巨噬细胞杀伤能力的实验研究  

Research of the differences of escaping killing by macrophage between breast cancer cell lines

作　　者：鄢艳红 孙释然 王龙强[2] 黄韬[2] 

机构地区：[1]湖北省中山医院神经内科,湖北武汉430032 [2]华中科技大学同济医学院附属协和医院乳腺甲状腺外科中心,湖北武汉430022

出　　处：《中国现代普通外科进展》2014年第10期768-770,共3页Chinese Journal of Current Advances in General Surgery

摘　　要：目的:研究乳腺癌细胞株MCF-7和MDA-MB-231逃避巨噬细胞杀伤能力的差异。方法:将巨噬细胞上清液分别加入MCF-7和MDA-MB-231细胞培养液中培养48 h,再加入CCK-8孵育,使用酶标仪检测吸光度值。结果:MDA-MB-231细胞存活率高于MCF-7细胞[(124.03±4.86)%vs(44.22±1.38)%],差异有统计学意义(P<0.05)。结论:巨噬细胞对肿瘤细胞的作用与肿瘤细胞的分子分型有关,MDA-MB-231细胞株较MCF-7细胞株有更强逃避巨噬细胞杀伤的能力。Objective：To study the difference of escaping killing by macrophage between MCF-7 and MDA-MB-231 in vitro.Methods：Add the macrophages that respectively supernatant fluid MCF-7 and MDA-MB-231 cells culture in 48 h,then add CCK 8 incubation,enzyme standard instrument were used to detect absorbance values.Results：The survival rate of MDA-MB-231 was higher than that of MCF-7 culturing with supernatant of macrophage（124.03％ ± 4.86％ to 44.22％ ± 1.38％,P＜0.05）.Conclusion：The role of macrophages in tumor cells related to the molecular classification of tumor cells.Our results suggest that MDA-MB-231 cells display stronger ability to escape killing by macrophage.

关 键 词：乳腺肿瘤 巨噬细胞 MCF-7细胞 MDA-MB-231细胞 

分 类 号：R737.9[医药卫生—肿瘤]