血管内皮祖细胞移植治疗大鼠缺血性脑损伤的实验研究  

Transplantation of endothelial progenitor cells in the treatment of cerebral ischemia in rats

作　　者：徐丽艳[1] 侯唯姝[1] 余永强[1] 

机构地区：[1]安徽医科大学第一附属医院放射科,安徽合肥230022

出　　处：《实用放射学杂志》2014年第12期2072-2075,共4页Journal of Practical Radiology

基　　金：安徽省自然科学基金（11040606Q22）。

摘　　要：目的：利用 MRI 活体示踪法联合神经功能评分（NSS）、病理学分析共同探讨大鼠胃髓源性内皮祖细胞（EPCs）在大鼠脑缺血损伤区促进神经功能恢复及新生血管形成中的作用。方法分离、培养、鉴定大鼠骨髓源性 EPCs 并行超小超顺磁性氧化铁颗粒（USPIO）磁性标记。大鼠大脑中动脉阻塞（tMCAO）模型30只,随机分为2组,分别经尾静脉移植 USPIO 标记 EPCs（实验组）及生理盐水（对照组）,于大鼠造模成功后第0、2、4、6、8 d 行 NSS 和 MRI 动态扫描,8 d 后行普鲁士蓝染色、微血管密度（MVD）测定。结果MR T2 WI 序列扫描于脑梗死区观察到低信号,普鲁士蓝染色结果与体外细胞染色结果相符。NSS 和 MVD 测定显示实验组大鼠脑缺血损伤区神经功能和血管密度高于对照组,差异有统计学意义。结论MRI 动态示踪、NSS 及病理学等多种方法证实了 EPCs 能够“归巢”至脑缺血性损伤区发挥作用,这一发现可能为缺血性脑血管疾病的干细胞移植治疗提供较好的多能成体干细胞来源。Objective To explore the role of endothelial progenitor cells （EPCs）in improving functional recovery and promoting neurogenesis in damaged area of ischemic rat brain by MRI,neurological severity score （NSS）,and pathological examinations.Meth-ods EPCs wereseparated,cultured,and identified,ultrasmall superparamagnetic iron oxide（USPIO）particles was used to label EPCs in vitro.Transient middle cerebral arterial occlusion（tMCAO）was successfully performed in 30 adult SD rats.Magnetically labeled cells（the experimental group）and normal saline （the control group）were injected intravenously into the tMCAO rats through the tail vein respectively,then MR imaging and NSS was performed 0 day,2 days,4 days,6 days and 8 days later.At last the paraffin-embedded rat brain tissues were obtained and examed by Prussian blue staining and Measurement of microvessel density.Re-sults EPCs wereseparated,cultured,and identified successfully.MR imaging showed significant low signal intensity at the ischemic area on T2 WI sequence.Prussian blue staining images were corresponding to the EPCs staining in vitro.The NSS and microvessel density in experimental group were significantly higher than in control group.Conclusion EPCs can migrate to the damage zone, improve functional recovery and promote neurogenesis in damaged area of ischemic rat brain,and may be a new source of multipoten-tial stem cells for stroke treatment.

关 键 词：缺血性脑血管疾病 磁共振成像 内皮祖细胞 

分 类 号：R743[医药卫生—神经病学与精神病学] R445.2[医药卫生—临床医学]