μ、κ、δ阿片受体及酪氨酸羟化酶在MN9D细胞中的表达  

Expression of μ, κ and δ-opioid receptors and tyrosine hydroxylase in MN9D cells

作　　者：田鹏翔[1] 史为博[2] 王洁[2] 刘洁[2] 毕海涛[2] 马春玲 丛斌 

机构地区：[1]河北医科大学临床医学系,石家庄050017 [2]河北医科大学基础医学院法医学系,石家庄050017 [3]不详

出　　处：《解剖学杂志》2014年第6期751-754,共4页Chinese Journal of Anatomy

基　　金：国家级大学生创新创业训练计划项目（201210089007）

摘　　要：目的：检测多巴胺能性MN9D细胞中是否存在μ、κ、δ 3种阿片类受体及多巴胺能神经元特异性标记物酪氨酸羟化酶（TH）的表达,为建立吗啡依赖MN9D细胞模型奠定基础.方法：培养MN9D细胞后,采用免疫荧光标记观察μ、κ、δ3种受体蛋白表达状态;RT-PCR检测μ、κ、δ3种阿片类受体的mRNA表达;TH免疫荧光标记,观察多巴胺能神经元的表达数量.结果：免疫荧光及PCR结果均显示MN9D细胞中存在μ、κ、δ 3种受体,并且TH阳性细胞的数目明显多于多巴胺能SH-SY5Y细胞系.结论：MN9D细胞中同时存在μ、κ、δ3种阿片受体,可作为吗啡依赖细胞模型使用.Objective：To detect the expression of μ,κ and δ-opioid receptors and tyrosine hydroxylase （TH）,a specific marker of dopaminergic neurons,in dopaminergic MN9D cells.The findings were meaningful to establish the cell model of morphine dependence in MN9D.Methods：Immunofluorescence staining and RT-PCR were used to observe protein and gene expression of μ,κ and δ-opioid receptors in MN9D cells.With immunofluorescence staining of TH,the quantity of dopaminergic neurons was studied.Results：Immunofluorescence and PCR results showed μ,κ and δ-receptors all existed in MN9D cells.And TH positive cells in MN9D cells were significantly more than those in SH-SY5Y cell lines.Conclusion：MN9D cells,owning μ,κ and δ-opioid receptors,can be used to establish morphine dependent cell models.

关 键 词：MN9D细胞 阿片受体 酪氨酸羟化酶 SH-SY5Y细胞 

分 类 号：R742.5[医药卫生—神经病学与精神病学]