芡实组培快繁无菌体系的建立和优化  

Studies on Optimization of the Conditions in Rapid Plantlet Regeneration of Euryale Ferox

作　　者：李彦连[1] 张华丽[2] 赵强[1] 魏晓丽[1] 王艳新[1] 张希才[1] 

机构地区：[1]济宁学院生命科学与工程系,山东曲阜273155 [2]济宁医学院药学院,山东日照276826

出　　处：《济宁学院学报》2014年第6期11-13,共3页Journal of Jining University

基　　金：山东省高等学校科技计划项目(J12LE59);济宁学院服务济宁重点项目(2009QNKJ09)

摘　　要：以芡实的单芽茎段为外植体进行离体培养和植株再生的优化研究.结果表明,以MS+6-BA1.5mg/L+NAA 0.2mg/L作增殖继代培养基为宜,以在MS培养基+蔗糖20g/L+琼脂5.0g/L中添加6-BA(0.1mg/L)+NAA(0.1mg/L)作生根培养基为宜,生根率最高.适当浓度的6-BA对芡实的不定芽增殖有明显的促进作用,6-BA和NAA配合使用可促进芽苗生长,对改善芡实植株组培苗质量有较好的作用.Stern segments with a single bud of Euryale ferox were used as explants in culture in vitro and for rapid propagation.The results indicated that MS＋ 6-BA 1.5 mg / L＋NAA 0.2 mg / L was a suitable medium for shoot multiplication,and the best medium for rooting was MS＋ 6-BA（0.1mg/L）＋NAA（0.1mg/L） used in proper concentrations promoted shoot multiplication but inhibited stem elongation.Combined application of 6-BA and NAA promoted the elongation of the stem segments.

关 键 词：芡实 组织培养 快速繁殖 优化 

分 类 号：S603[农业科学—园艺学]