三尖杉内生真菌S19次生代谢产物的结构与活性  

Structure and Activity of Secondary Metabolites of Endophytic Fungus S19 Strain in Cephalotaxus fortunei

作　　者：马聪聪[1] 马养民[1] 李婷[1] 王珺[1] 

机构地区：[1]陕西科技大学教育部轻化工助剂化学与技术重点实验室,陕西西安710021

出　　处：《贵州农业科学》2014年第12期152-156,共5页Guizhou Agricultural Sciences

基　　金：高等学校博士学科点专项科研基金(20116125110001);陕西省自然科学基础研究计划项目(2014JZ003)

摘　　要：为探明三尖杉内生真菌S19的次生代谢产物的结构与活性,采用形态学特征和ITS序列鉴定菌株,利用硅胶柱层析、Sephadex LH-20柱层析和重结晶等方法对该菌株的发酵产物进行分离、纯化,并结合所得化合物的理化性质和波谱数据鉴定其结构,通过测试菌对其次生代谢产物进行活性测试。结果表明:菌株S19为黄曲霉(Aspergillus flavus),从其发酵产物中分离得到6个化合物,分别为5-羟甲基呋喃-3-羧酸(1)、麦角甾醇(2)、β-谷甾醇(3)、豆甾-7,22-二烯-3β,5α,6α-三醇(4)、胶霉毒素(5)和丁二酸(6)。黄曲霉(S19)可以大量产生5-羟甲基呋喃-3-羧酸(1)。化合物1对大肠杆菌抑菌活性较好,其MIC值为15.6μg/mL;化合物5对测试菌均有很好的抑菌活性。The fermentation product of endophytic fungus S19 strain in C.fortunei identified by morphological feature and ITS sequence was isolated and purified by SGCCT,Sephadex LH-20 column chromatography and recrystallization methods,then their structures were identified according to their physicochemical property and spectrum data,and finally the activity of secondary metabolites was determined by testing bacteria.The results showed that S19 strain is identified as Aspergillus flavus,and six compounds of 5-hydroxymethyl furfuran-3-carboxylic acid,ergosterol,β-sitosterol,stigmasta-7,22-diene-3β,5α,6α-triol,gliotoxin and succinic acid are isolated from its fermentation product.5-hydroxymethyl furfuran-3-carboxylic acid has good bacteriostatic activity with MIC value of 15.6μg/mL against Escherichia coli.Gliotoxin is of good bacteriostatic activity against four testing bacteria of Pseudomonas aeruginosa,Escherichia coli,Staphylococcus aureus and Streptococcus lactis.

关 键 词：三尖杉 内生真菌 黄曲霉 次生代谢产物 抗菌活性 

分 类 号：S567.1[农业科学—中草药栽培] R284.1[农业科学—作物学]