两株好氧氨氧化菌(AOB)的分离纯化及菌属鉴定  

Isolation,Purification and Species Identification of Aerobic Ammonia-Oxidizing Bacteria( AOB)

作　　者：曾涛涛[1] 李冬[2] 曾辉平[2] 仲航[3] 张杰[2,4] 

机构地区：[1]南华大学污染控制与资源化技术湖南省高校重点实验室,湖南衡阳421001 [2]北京工业大学水质科学与水环境恢复工程北京市重点实验室,北京100124 [3]北京三磊建筑设计有限公司,北京100048 [4]哈尔滨工业大学城市水资源与水环境国家重点实验室,黑龙江哈尔滨150090

出　　处：《南华大学学报（自然科学版）》2014年第4期23-27,共5页Journal of University of South China：Science and Technology

基　　金：国家自然科学基金资助项目(51408293);中国博士后基金资助项目(2014M562114);南华大学博士科研启动基金资助项目(2013XQD10)

摘　　要：在生物脱氮过程中,好氧氨氧化细菌（ AOB）发挥重要作用.本研究通过梯度稀释培养、平板划线分离进行AOB分离纯化,获得两株氨氧化能力较强的AOB菌株A2和A7,它们能将培养液中的大部分氨氮氧化.对A2和A7菌株进行革兰氏染色,并通过光学显微镜及扫描电镜观察细胞形态,发现A2和A7均为革兰氏阴性菌,细胞呈杆状,长为1~1.5μm,宽约0.5μm.生理生化实验表明,A2和A7属于自养微生物,不能利用有机碳源.系统发育分析表明,A2和A7均为亚硝化单胞菌（ Nitrosomonas）.Aerobic ammonia_oxidizing bacteria （AOB） play important roles in biological nitrogen removal process. In this study,gradient dilution of domestication liquid and streak_ ing plate methods were used for AOB isolation and purification. Two strains of AOB,named A2 and A7,were obtained from culture medium,which contributed most ammonia oxida_ tion. After gram stain,detected by optical microscopy and scanning electron microscopy,A2 and A7 were both found as gram_negative and rod_shaped bacteria,with length of 1 ~1. 5 μm and width of 0. 5 μm. A2 and A7 were autotrophic microorganisms since organic car_ bon couldn’t be ultized. Phylogenetic analysis showed that the species of A2 and A7 were Nitrosomonas.

关 键 词：好氧氨氧化菌 分离 鉴定 生物脱氮 

分 类 号：X172[环境科学与工程—环境科学]