外源性拮抗神经纤毛蛋白1促进人舌癌细胞SCC9细胞凋亡的研究  

Exogenous inhibition of Neuropilin-1 enhance the apoptosis of human tongue squamous cell carcinoma cell line SCC9

作　　者：查霖 吴煜农[2,3] 郑灏[1] 宋晓萌[2,3] 唐子春[1] 

机构地区：[1]铜陵市人民医院口腔科,安徽铜陵244000 [2]南京医科大学口腔疾病研究江苏省重点实验室,江苏南京210029 [3]南京医科大学附属口腔医院颌面外科,江苏南京210029

出　　处：《口腔生物医学》2014年第4期185-188,共4页Oral Biomedicine

基　　金：安徽省铜陵市卫生科研项目(2014-26)

摘　　要：目的:研究外源性拮抗人舌癌细胞SCC9中神经纤毛蛋白1(Neuropilin-1,NRP-1)蛋白对人舌癌细胞SCC9增殖、凋亡的影响。方法:通过免疫印迹实验(western blot,WB)及实时定量荧光PCR(real-time RT-PCR)分别从蛋白水平及mRNA水平检测小分子肽ATWLPPR(A7R)对SCC9细胞NRP-1表达的影响,利用细胞计数法及流式细胞术检测拮抗NRP-1蛋白后对SCC9细胞增殖、凋亡的影响。结果:A7R拮抗后SCC9细胞NRP-1的mRNA水平下降而蛋白表达未见明显下降,同时对SCC9细胞凋亡有促进作用,而对SCC9细胞增殖无明显影响。结论:外源性拮抗NRP-1可促进SCC9细胞凋亡。Objective：To investigate the effect on the proliferation and the apoptosis of the human tongue squamous carcinoma cell line SCC9 through exogenous inhibition of Neuropilin-1 （NRP-1 ）.Methods：Western blot and real-time RT-PCR were employed to ex-amine the effect on the expression of NRP-1 in cell line SCC9 exogenous inhibition of NRP-1 .Besides,cell counting and flow cytometry （FC）methods were used to examine the influence of the proliferation and the apoptosis of the SCC9 cells exogenous inhibition of NRP-1 .Results：After the inhibition,mRNA level of NRP-1 decreased,whereas the expression of protein level of NRP-1 did not decrease obviously.Exogenous inhibition of NRP-1 enhanced the apoptosis of SCC9 cells while rarely affected the proliferation of SCC9 cells. Conclusions：Exogenous inhibition of NRP-1 enhances the apoptosis of the SCC9 cells.

关 键 词：神经纤毛蛋白 1 拮抗 舌癌 细胞凋亡 NRP-1 

分 类 号：R739.9[医药卫生—肿瘤]