赣南地区非综合征性唇腭裂患者尿液代谢组学的初步研究  

The Preliminary Investigation on Urine of Non-syndromic Cleft Lip and/or Palate(NSCL/P)Using NMR-based Metabonomics in Southern Jiangxi

作　　者：凌永昌[1] 刘瑞珍[2] 刘翔[1] 张莹[1] 刘新征[1] 李年生[1] 

机构地区：[1]赣南医学院第一附属医院,江西赣州341000 [2]赣南医学院

出　　处：《中国医学创新》2014年第34期105-107,共3页Medical Innovation of China

基　　金：赣州市指导性科技计划项目(2013025)

摘　　要：目的:通过检测非综合征性唇腭裂(NSCL/P)患者与正常人尿液中代谢物的变化,探讨基于核磁共振(NMR)的代谢组学在此症的临床应用中的可行性。方法:分别收集12个NSCL/P患者和12个正常人对照组的尿液样品。采用NMR分析NSCL/P患者尿液代谢特征,测定1 H-NMR图谱,进而用主成分分析法(PCA)处理NMR数据,研究NSCL/P组和正常对照组尿液中的代谢物差异,获得NSCL/P患者的特异性代谢标志物。结果:NSCL/P组和正常对照组间的尿液代谢产物存在明显差别。结论:采用基于NMR的代谢组学技术能较好地反映NSCL/P患者尿液的代谢变化,获得相应的特征代谢物,在未来的临床应用中具有广阔的前景。Objective： To detect the metabolites in urine of non-syndromic cleft lip and/or palate（NSCL/P） patients and to explore the feasibility of employing nuclear magnetic resonance（NMR）-based metabonomics in clinical practice.Method： Urine samples were collected from 12 NSCL/P patients group and 12 healthy control group. Urine metabolomic profiles were assessed by 1 H-NMR spectroscopy and principal component analysis（PCA）. Discriminating metabolites were identified, and metabonomic differences of urine were examined between NSCL/P patients group and the control group. Result： The urine metabolic profiling in NSCL/P patients group was remarkably distinct from that of healthy control group. Conclusion： The application of NMR-based metabonomics techniques reflected urine metabolic changes of patients with NSCL/P,indicating the great potential of metabonomics in clinic diagnosis and screening.

关 键 词：非综合征性唇腭裂 基于核磁共振的代谢组学 主成分分析 

分 类 号：R782.2[医药卫生—口腔医学]