蛋白激酶C抑制剂对偏头痛大鼠三叉神经脊束核细胞外调节蛋白激酶磷酸化的影响  

Effect of protein kinase C inhibitor on the level of phosphralated extracellular regulated protein kinases in the spinal trigeminal nucleus of migraine model rats

作　　者：张家君[1] 杨明峰[3] 孙保亮[3] 张继国[2] 张颜波[3] 

机构地区：[1]泰山医学院附属医院超声科,泰安271016 [2]泰山医学院附属医院药学院,泰安271016 [3]泰山医学院附属医院附属医院神经内科,泰安271016

出　　处：《中华行为医学与脑科学杂志》2014年第12期1072-1074,共3页Chinese Journal of Behavioral Medicine and Brain Science

基　　金：国家科技支撑计划项目（2013BA107801）;山东省自然科学基金项目（ZR2012HQ014,ZR2011HM044）

摘　　要：目的 探讨蛋白激酶C（PKC）抑制剂H-7对偏头痛大鼠三叉神经脊束核细胞外调节蛋白激酶（ERK1/2）磷酸化的影响.方法 SD大鼠随机数字表法分为：正常组（N组）、假手术组（C组）、偏头痛模型组（M组）和PKC抑制剂H-7组（H-7组）,每组n=18.进行硬脑膜血流量、三叉神经脊束核神经元放电记录和ERK1/2磷酸化水平检测.结果 （1）硬脑膜血流量检测：造模后2hM组[（78.0±4.2）％]与C组[（3.8±1.0）％]相比明显增加,差异有统计学意义（P＜0.01）;H-7组[（-24.8±4.9）％]与M组[（78.0±4.2）％]相比明显减少,差异有统计学意义（P＜0.01）.（2）放电变化：造模后三叉神经脊束核细胞外放电增加,造模后2h为造模前的[（325.9±47.3）％];M组[（325.9±47.3）％]高于C组[（107.3±16.4）％]（P＜0.01）.H-7组[（136.0±26.5）％]低于M组[（325.9±47.3）％]（P＜0.01）;H-7组C相比,差异无统计学意义（P＞0.05）.（3）磷酸化水平：M组ERK1/2磷酸化水平高于C组（P＜0.01）;H-7组低于C组（P＜0.05）;H-7组低于M组（P＜0.01）.结论 在偏头痛中枢发病机制中,ERK1/2是PKC下游信号通路,PKC可能对ERK1/2具有间接激活作用.Objective To explore the effect of protein kinase C inhibitor on the level of phosphralated extracellular regulated protein kinases in the spinal trigeminal nucleus of migraine model rats.Methods Healthy adult male SD rats were randomly divided into four groups：normal group （group C）,sham operation group （group C）,migraine model group（group M）,and H-7group（H-7group）,with 18 rats in each group.Dural blood flow and the extracellular discharge frequency in the spinal trigeminal nucleus was recorded.ERK1/2 phosphorylation was tested.Results （1） Dural blood flow：compared with group C（（3.8± 1.0）%）,the dural blood flow in M group （（78.0±4.2） %）increased obviously（P〈0.01） ; compared with M group（（78.0±4.2）%）,the dural blood flow in H-7 group（（-24.8±4.9） %） decreased obviously（P〈0.01）.（2） The percentage of extracellular discharge frequency change：two hours after treatment,the percentage of extracellula discharge frequency change in group M （（325.9 ±47.3）%）was higher than that in group C（（107.3±16.4）%）.The percentage of discharge frequency change in group H-7（（136.0±26.5）%） was lower than that in group M（（325.9±47.3）%）.There was no significant difference in the percentage of discharge frequency change between group H-7（（136.0±26.5） %） and group C（（107.3 ± 16.4）%）.（2） ERK1/2 phosphorylation：the ERK1/2 phosphorylation in group M was higher than that in group C.The ERK1/2 phosphorylation in group H-7 was lower than than that in group C and group M.Conclusion ERK1/2 is a downstream PKC signal path and PKC may have indirect activation of ERK1/2.

关 键 词：偏头痛 ERK1/2 中枢敏感化 三叉神经脊束核 蛋白激酶C 

分 类 号：R747.2[医药卫生—神经病学与精神病学]