槲皮素对慢性混合反流性食管炎大鼠食管黏膜的保护作用及对核转录因子-κB/白细胞介素-6信号通路的影响  

Protective effects of quercetin on esophageal mucosa in chronic mixed reflux esophagitis rats and its effect on nuclear factor-κB/interleukin-6 signaling pathway

作　　者：吴萍[1] 罗斌[1] 李莹杰[1] 周璐[2] 易丽莎[2] 陈胜芳[1] 许树长[1] 

机构地区：[1]同济大学附属同济医院临床营养科,上海200065 [2]同济大学附属同济医院消化科,上海200065

出　　处：《中华临床营养杂志》2014年第6期360-367,共8页Chinese Journal of Clinical Nutrition

基　　金：国家自然科学基金（81370491）;上海市卫生与计划生育委员会课题（WSJ1318）

摘　　要：目的 观察槲皮素对慢性混合反流性食管炎大鼠食管黏膜损伤的保护作用及对核转录因子κB （NF-κB）/白细胞介素（IL）-6信号通路的影响.方法 采用贲门结扎术＋食管十二指肠吻合术建立慢性混合反流性食管炎大鼠模型.48只健康清洁级SD雄性大鼠采用随机数字表法分为正常对照组、假手术组、模型对照组、奥美拉唑组、低剂量槲皮素组和高剂量槲皮素组,每组8只,分别腹腔注射生理盐水2 ml（正常对照组、假手术组和模型对照组）、奥美拉唑20 mg/kg（奥美拉唑组）、槲皮素100 mg/kg（低剂量槲皮素组）和槲皮素200 mg/kg（高剂量槲皮素组）,每日1次.6周后处死,光镜下观察食管组织的形态学改变并评分,分别采用免疫组织化学染色及酶联免疫吸附试验检测NF-κB p65、IL-6蛋白在大鼠食管黏膜及血清中的表达,各组进行比较.结果 正常对照组、假手术组、模型对照组、奥美拉唑组、低剂量槲皮素组、高剂量槲皮素组大鼠的食管黏膜病理评分分别为0.250±0.463、0.250±0.463、2.625±0.518、1.500±0.535、1.250±0.463、1.375±0.518;食管黏膜NF-κB p65蛋白评分分别为0.500±0.535、0.625±0.518、3.500±0.535、1.875±0.649、1.750±0.707、2.000±0.535;食管黏膜IL-6蛋白评分分别为1.125±0.641、1.125±0.835、5.375±0.518、2.375±0.518、2.000±0.535、2.250±0.463;血清NF-κB p65蛋白含量分别为（68.618±18.500）、（77.824±22.228）、（184.882±49.165）、（106.693±45.312）、（76.215±16.588）、（108.207±42.107） pg/ml;血清IL-6蛋白含量分别为（24.826±4.008）、（23.599±4.351）、（51.378±9.697）、（32.370±11.657）、（23.085±4.660）、（26.243±4.955） pg/ml.假手术组与正常对照组比较,以上5项指标差异均无统计学意义（P=1.000、P=0.642、P=1.000、P=0.518、P=0.673）;正常对照组、假手术组、奥美拉唑组、低剂量槲皮素组和高剂量槲皮素组与模型对照组比�Objective To observe the protective effects of quercetin on esophageal mucosa in chronic mixed reflux esophagitis （RE） rats and the effect of quercetin on nuclear factor （NF）-κB/interleukin （IL）-6 signaling pathway.Methods Mixed RE model was successfully induced by cardia ligation and esophagoduodenostomy.48 healthy male Sprague-Dawley rats were equally divided into the following 6 groups using random number table method：normal control group,sham-operation group,model control group,omeprazole group,low-dose quercetin group,and high-dose quercetin group.The 6 groups were treated with peritoneal injection of 2 ml normal saline （normal control,sham-operation,model control groups）,20 mg/kg omeprazole,100 mg/kg quercetin （low-dose） and 200 mg/kg quercetin （high-dose） once daily,respectively.The rats were sacrificed after 6 weeks of intervention.The microscopic pathological changes of esophageal mucosa were scored.NF-κB p65 and IL-6 protein levels in esophageal mucosa and serum were assessed using immunohistochemistry and enzyme-linked immunosorbent assay,respectively.Results In normal control group,shamoperation group,model control group,omeprazole group,low-dose quercetin group and high-dose quercetin group,the pathological scores of esophageal mucosa were 0.250 ± 0.463,0.250 ± 0.463,2.625 ± 0.518,1.500 ±0.535,1.250 ±0.463,and 1.375 ±0.518; the NF-κB p65 protein scores in esophageal mucosa were 0.500±0.535,0.625 ±0.518,3.500 ±0.535,1.875 ±0.649,1.750 ±0.707,and 2.000 ±0.535; the IL-6 protein scores in esophageal mucosa were 1.125 ± 0.641,1.125 ± 0.835,5.375 ± 0.518,2.375 ± 0.518,2.000 ±0.535,and 2.250 ±0.463; the serum NF-κB p65 protein levels were （68.618 ± 18.500）,（77.824 ± 22.228）,（184.882 ± 49.165）,（106.693 ± 45.312）,（76.215 ± 16.588）,and （108.207 ± 42.107） pg/ml; the serum IL-6 protein levels were （24.826 ±4.008）,（23.599 ±4.351）,（51.378 ± 9.697）,（32.370 ± 11.657）,（23.085 ± 4.660）,and （26.243 ± 4.955） pg/ml

关 键 词：槲皮素 反流性食管炎 核转录因子-ΚB 白细胞介素-6 

分 类 号：R571[医药卫生—消化系统]