乳腺癌髓系来源抑制细胞中吲哚胺2,3-双加氧酶表达与Tregs分布关系及临床意义  

Relationship and Clinical Significance between Myeloid Suppressor Cells in Indoleamine 2,3- Expression and Distribution of Tregs Dioxygenase in Breast Cancer

作　　者：黄亚琼[1] 赵庆丽[1] 张锋军[1] 顾丽萍[1] 

机构地区：[1]中国人民解放军兰州军区总医院乳腺科,甘肃兰州730050

出　　处：《河北医学》2015年第1期134-137,共4页Hebei Medicine

基　　金：甘肃省卫生厅科研基金;(编号:2012D59)

摘　　要：目的:研究乳腺癌CD33+髓系来源的抑制细胞(MDSCs)中吲哚胺2,3-双加氧酶(IDO)表达情况及与Foxp3+调节性T细胞(Tregs)分布关系和临床意义。方法:运用免疫组化单染技术检测CD33+MDSCs细胞、Foxp3+Tregs,采用免疫组化双染检测CD33+MDSCs细胞中IDO的表达情况。结果:癌组织中CD33+MDSCs细胞、Foxp3+Tregs分布无序,呈散在分布;Foxp3+Tregs高比例组中,CD33+MDSCs细胞中IDO呈高表达,差异有统计学意义(P<0.05);CD33+MDSCs中IDO的表达与患者年龄、肿瘤直径和组织学分级无明显关系,但与是否有淋巴结转移有密切关系(P<0.05)。结论:乳腺癌中CD33+MDSCs中IDO高表达参与肿瘤细胞免疫耐受的形成,可能与乳腺癌淋巴转移存在密切联系。Objective：To study the expression of indoleamine 2,3-dioxygenase （ IDO） in CD3^3＋mye-loid-derived suppressor cells （ MDSCs） and the relationships between the expression of IDO and Foxp^3＋reg-ulatory T cells （ Tregs） distribution and clinical significance. Method：Immunohistochemical single staining technique was used to detect CD3^3＋MDSCs cells and Foxp3＋Tregs.The immunohistochemical double staining was used to detect CD3^3＋MDSCs cells indoleamine 2,3-bis dioxygenase （IDO） expression.Result：Carci-noma CD3^3＋MDSCs cells, Foxp3＋Tregs distribution were disorder and scattered in carcinoma. It showed sig-nificant difference between the expression of MDSCs IDO and Tregs distribution （ P〈0.05）. The expression of CD3^3＋MDSCs in IDO had no relation with breast cancer , irrespective of age , tumor size and histological grade were coreelated with whether lymph node metastasis （P〈0.05）.Conclusion：It showed a close contact between carcinoma CD 33＋MDSCs IDO expression in tumor cells and the formation of immune tolerance. Carcinoma CD3^3＋MDSCs IDO expression may be associated with the presence of lymph node metastasis.

关 键 词：乳腺癌 吲哚胺2 3-双加氧酶 免疫耐受 CD3^3+MDSCs Foxp^3+Tregs 

分 类 号：R737.9[医药卫生—肿瘤]