不同提取工艺对鄂产绿茶多糖体外降糖活性的影响  

Influence of Different Extraction Technologies on Hypoglycemic Activity of Hubei Green Tea Polysaccharide in Vitro

作　　者：贾亮亮[1,2] 奚炜[1,2] 彭官良[1,2] 曾晓[1,2] 陈进兵[1,2] 金桂兰[1,2] 

机构地区：[1]三峡大学人民医院 [2]宜昌市第一人民医院药学部,宜昌443000

出　　处：《医药导报》2015年第1期7-10,共4页Herald of Medicine

基　　金：宜昌市科技研究与开发项目(A12301-21)

摘　　要：目的探讨不同提取方法对鄂产绿茶多糖降糖活性的影响。方法分别采用酶法、热水提取法、冷水提取法提取鄂产绿茶的茶多糖。通过体外实验观察不同提取方法所得绿茶多糖对α-葡萄糖苷酶、α-淀粉酶、蔗糖酶活性影响,选出对酶抑制作用最强的多糖。结果 3种提取方法所得绿茶多糖对α-葡萄糖苷酶的抑制作用无明显差别;酶法所提绿茶多糖对α-淀粉酶、蔗糖酶活性强于热水提取法、冷水提取法所得的绿茶多糖。结论酶法提取的绿茶多糖具有较好的体外降血糖活性。Objective To investigate the influence of different extraction methods on hypoglycemic activities of polysaccharides from Hubei green tea. Methods Different green tea polysaccharides were extracted by enzymatic extraction, hot water extraction or cold water extraction, respectively. The inhibitory activities of the extract on o^-glueosidase, oL-amylase and invertase in vitro were investigated to determine the most efficient extraction technique. Results There was no significant difference in the inhibition of ct-glucosidase by polysaceharides from three different extractions. The polysaccharides extracted by enzyme method were more active on a-amylase and invertase than those extracted from hot water and cold water methods. Conclusion The green tea polysaccharides extracted by enzyme method showed better hypoglyeemic activity in vitro.

关 键 词：绿茶多糖 提取工艺 降糖活性 

分 类 号：R282.71[医药卫生—中药学] R285[医药卫生—中医学]