肝细胞生长因子对人胃癌细胞株AGS增殖的促进作用  

Hepatocyte Growth Factor Enhances the Proliferation of Human Gastric Cancer Cell Line AGS

作　　者：凌跃新[1] 童赛雄[1] 

机构地区：[1]复旦大学附属中山医院普外科,上海200032

出　　处：《中国临床医学》2014年第5期529-530,共2页Chinese Journal of Clinical Medicine

摘　　要：目的:肝细胞生长因子(hepatocyte growth factor,HGF)是一种多肽生长因子,具有促进细胞增殖、诱导细胞迁移和形态改变的作用。本研究探讨HGF对人胃癌细胞株AGS增殖的影响。方法:体外培养人胃癌细胞株AGS,应用浓度为25、50、75、100 ng/mL的HGF分别处理AGS细胞24 h、48 h、72 h,采用CCK-8法检测AGS细胞的增殖情况。结果:CCK-8法检测结果显示,不同浓度的HGF对AGS细胞均有促增殖作用,随着HGF浓度的增加或作用时间的延长,细胞增殖活力呈上升趋势,在72 h最为明显,呈时间、浓度依赖关系(t为3.89、5.28、9.73、11.00,P<0.01)。结论:HGF能显著促进人胃癌细胞株AGS的增殖。Objective：Hepatocyte growth factor （HGF）is a polypeptide growth factor with effects on the cell proliferation, motility,and morphogenesis.The purpose of this research was to explore the effect of HGF on the proliferation of human gas-tric cancer cell line AGS.Methods：Human gastric cancer cell line AGS was cultured in vitro.AGS cells were treated with 25, 50 ,75 and 100ng/mL of HGF for 24 h,48 h and 72 h,respectively.Cell proliferation was measured by CCK-8 assay.Results：CCK-8 assay result showed that compared with the control,all of the four concentrations of HGF could increase the prolifera-tion of AGS cells.With the increase of HGF concentration and treating time,the proliferation activity of AGS cells was the up-ward trend in a dose-and time-dependent manner （t=3.89,5.28,9.73,11.00,P 〈0.01）.Conclusions：HGF can significant-ly promote the proliferation of human gastric cancer AGS cells.

关 键 词：肝细胞生长因子 人胃癌细胞 增殖 

分 类 号：R735.2[医药卫生—肿瘤]