高效液相色谱法测定葡萄酒中苋菜红的不确定度评定  

Uncertainty Evaluation for the Determination of Amaranthus Red in Wine Samples by High Performance Liquid Chromatography

作　　者：马康[1] 李小佳[2] 崔萌萌[3] 马联弟[1] 

机构地区：[1]中国计量科学研究院,北京100029 [2]北京科技大学化学与生物工程学院,北京100083 [3]北京化工大学化学理学院,北京100029

出　　处：《计量学报》2015年第1期102-106,共5页Acta Metrologica Sinica

基　　金：科技部公益性行业科研专项(2012104001);科技基础性工作专项(2008FY130200);中国计量科学研究院基础科研业务费(21-AKY1216)

摘　　要：通过对高效液相色谱法测定葡萄酒中合成色素苋菜红质量浓度的全过程分析，确定了测定结果不确定度的来源。采用计量学不确定度传递模型，对引入的不确定度分量进行评定，确定了葡萄酒中苋菜红质量浓度标准不确定度由标准物质、标准溶液配制、标准曲线拟合、样品制备、测量重复性和回收率等6部分不确定度合成得到。2个不同质量浓度样品测定结果不确定度被评定，其相对扩展不确定度分别为2．6％和2．2％（k=2），由不确定度评价结果分析得到，样品重复性测定和标准溶液配制是测定结果不确定度的主要来源。Sources of measurement for amaranthus red in wine samples by high performance liquid chromatography are determined through studying the whole process of analytical results. The uncertainties of analytical result are evaluated by a metrological model of uncertainty. The standard uncertainty for analytical results for amaranthus red in wine samples is mainly consisted of uncertainty of solution reference material of amaranthus red, preparation of work standard solution, calibration curve using the least squares method, preparation for real wine sample, measured repeatability, method recovery and so on. Relative expanded uncertainties for two real wine sample with different content are evaluated and they are 2. 6% and 2.2% （k = 2）. Evaluation of analytical results of two samples with different content indicated that main resource of uncertainty are measured repeatability and calibration curve.

关 键 词：计量学 苋菜红 葡萄酒 高效液相色谱 不确定度 

分 类 号：TB99[一般工业技术—计量学]