蟾毒噻咛的抗肿瘤作用  

Anti-tumor activity of bufothionine

作　　者：王舒雨 赵明蕊 王俊巍[2] 李云飞 岳小欣 

机构地区：[1]河南医学高等专科学校药学系,郑州451191 [2]郑州大学药学院新药研发中心,郑州450001

出　　处：《郑州大学学报（医学版）》2014年第5期671-674,共4页Journal of Zhengzhou University(Medical Sciences)

摘　　要：目的:研究蟾毒噻咛的抗肿瘤作用。方法:取3种肿瘤细胞株ECa109、HepG2、A549,每种细胞分为9组,分别加入0.125、0.250、0.500、1.000、2.000、4.000、8.000、16.000、32.000 mg/L的蟾毒噻咛处理48 h。采用MTT比色实验计算细胞增殖抑制率;采用流式细胞术检测HepG2细胞周期及凋亡。建立荷S180腹水瘤昆明小鼠模型,随机分为5组,每组20只,阴性对照组注射溶剂,阳性对照组每天给予20 mg/kg的5-FU,蟾毒噻咛低、中、高剂量组分别给予15、20和30 mg/kg的蟾毒噻咛,连续腹腔注射给药7 d。末次给药后每组随机抽取半数荷瘤小鼠,收集腹水,计算腹水抑制率和瘤细胞存活率;剩余小鼠统计生存时间,计算生命延长率。结果:随着药物质量浓度的增大,蟾毒噻咛对3种肿瘤细胞的抑制作用增强,其中对HepG2细胞的抑制作用最强(F=6.785、29.641和14.455,P<0.05);蟾毒噻咛能诱发HepG2细胞凋亡,将细胞周期阻滞在G2期。蟾毒噻咛还能提高荷瘤鼠腹水抑制率,抑制瘤细胞存活率,提高荷瘤鼠生命延长率(F=412.321、900.735和1 151.272,P<0.05)。结论:蟾毒噻咛具有抗肿瘤作用。Aim：To investigate the anti-tumor activity of bufothionine , which is an extract of toad skin .Methods：ECa109,HepG2,and A549 cells were used to assess the inhibitory effect of bufothionine .The cells were all allocated into 9 groups and treated with bufothionine at 0.125,0.250,0.500,1.000,2.000,4.000,8.000,16.000,and 32.000 μg/L.The inhibition effect of bufothionine was analyzed by MTT assay .The cell apoptosis and the cell cycle distribution ,by flow cy-tometry.Kunming mice bearing ascites tumor were allocated into 5 groups at random：control group,5-fluorouracil group[20 mg/（kg＆#183; d）],bufuthionine low-,middle-,and high-dose[30,20,15 mg/（kg＆#183; d）] groups.The treatments were given 7 times and the interval was 24 h.After the last treatment,a half mice were executed and the ascites were collected .Ascites inhibition rate and tumor cell persistence rate were calculated .The other half were used to observe the survival status .Re-sults：Bufothionine had dose-dependent inhibitive effects on the tumor cells especially on HepG 2（F=6.785,29.641,and 14.455,P〈0.05）.Bufothionine could induce cell apoptosis and arrest the cells at G 2 phase;bufothionine could inhibit the produce of ascites and the survival of tumor cells , and prolong the survival of mice bearing ascites tumor （ F=412 .321 , 900.735 and 1 151.272,P〈0.05）.Conclusion：Bufothionine has anti-tumor effects.

关 键 词：蟾毒噻咛 HEPG2 A549 ECA109 小鼠 

分 类 号：R730.5[医药卫生—肿瘤]