埃克替尼对ACC-M细胞凋亡的影响  

Effect of icotinib on apoptosis of ACC-M cells

作　　者：杨彩玲[1] 张景航[2] 任铭新[3] 张应花[3] 崔卫刚[3] 

机构地区：[1]新乡医学院第一附属医院口腔外科,卫辉453100 [2]新乡医学院病理科,新乡453003 [3]新乡医学院人体解剖学教研室,新乡453003

出　　处：《郑州大学学报（医学版）》2014年第5期681-683,共3页Journal of Zhengzhou University(Medical Sciences)

摘　　要：目的:研究埃克替尼对涎腺腺样囊性癌细胞系ACC-M细胞凋亡的影响及可能机制。方法:ACC-M细胞分为6组,即对照组,低、中、高剂量(10、20、40μmol/L)埃克替尼组,MnTMPyP组以及MnTMPyP+埃克替尼组。采用MTT法检测各组细胞的活性,Caspase-3活力检测试剂盒检测Caspase-3蛋白的活力,DCFH-DA荧光探针检测ROS水平,Western blot检测线粒体和胞质MnSOD蛋白的表达。结果:与对照组相比,埃克替尼低、中、高剂量组细胞活性降低,Caspase-3蛋白活力增强,ROS水平升高,线粒体中MnSOD蛋白表达减少,胞质中MnSOD蛋白表达增加(P均<0.05);MnTMPyP能拮抗上述变化(P均<0.05)。结论:埃克替尼可能通过ROS介导的MnSOD线粒体转位诱导ACC-M细胞凋亡。Aim：To observe the effect and mechanism of icotinib on the apoptosis induction of the salivary adenoid cystic carcinoma-M（ ACC-M） cells.Methods：ACC-M cells were allocated into control group ,low-,median-,high-concen-tration（10,20,40 μmol/L） icotinib-treatment groups,MnTMPyP group and MnTMPyP ＋icotinib group.The cell viability of ACC-M was measured by MTT assay .The expression of Caspase-3 was assessed by Caspase-3 activity detection kit .The lev-el of ROS was assayed by the fluorescent probe using DCFH-DA.The expression of MnSOD in mitochondrion was deter-mined by Western blot analysis .Results：Compared with control group ,the cell viability of ACC-M significantly decreased in low-,median-,high-concentration icotinib groups ,the expressions of Caspase-3 and ROS increased ,the expression of Mn-SOD significantly decreased in mitochondrion while increased in cytoplasm （all P〈0.05）.However,MnTMPyP reversed the effects of icotinib（all P〈0.05）.Conclusion：Icotinib may induce apoptosis of ACC-M cells through ROS-mediated Mn-SOD mitochondrial translocation .

关 键 词：埃克替尼 线粒体转位 凋亡 活性氧 ACC-M细胞 

分 类 号：R739.86[医药卫生—肿瘤]