2-吡唑酰壳低聚糖的制备、表征及其抗菌活性的研究  

Synthesis, Characterization and Antibacterial Activity of 2-pyrazole Acylation Oligo-chitosan

作　　者：赵迪[1] 董泽义[1] 王江涛[1] 孟祥红[2] 

机构地区：[1]中国海洋大学海洋化学理论和工程技术教育部重点实验室,山东青岛266100 [2]中国海洋大学食品科学与工程学院,山东青岛266100

出　　处：《高校化学工程学报》2014年第6期1415-1419,共5页Journal of Chemical Engineering of Chinese Universities

基　　金：国家自然科学基金(41076065)

摘　　要：以壳低聚糖与氯乙酰氯反应制备出中间产物2-氯乙酰壳低聚糖,中间产物再与吡唑反应得到2-吡唑酰壳低聚糖。通过FT-IR、1H-NMR、热重分析、溶解度实验对产物进行了结构和性能表征,并测定了产物对褐腐病菌和灰霉病菌的抑菌活性。结果表明:目标产物结构正确,热稳定性略低于壳低聚糖;在水中的溶解度为3 mg·m L-1;抑菌研究表明,产物的抑菌活性随浓度的增大而增大,对褐腐病菌菌丝生长抑制的IC50值为0.33 mg·m L-1,对灰霉病菌菌丝生长抑制的IC50为0.45 mg·m L-1,抑菌活性优于壳低聚糖。2-chloracetyl oligo-chitosan was obtained by the reaction of oligo-chitosan and chloroacetyl chloride, and then 2-pyrazole acylation oligo-chitosan was synthesized by the reaction of 2-chloracetyl oligo-chitosan and pyrazole. The performance and structure of the product were characterized by FT-IR, 1H-NMR, thermogravimetry （TG）, solubility and antibacterial activity analysis. The results showed that the derivative is the target product with its thermal stability inferior to oligo-chitosan. The solubility of derivate is 3 mg-mL-1 in water. The results indicate that the antimicrobial activitiy of the oligo-chitosan derivative increases with the increase of its concentration. It was also found that the antimicrobial activities of the derivative are stronger than those of oligo-chitosan, and the 50% inhibition concentrations of 2-pyrazole acylation oligo-chitosan for the growth of Moniliniafructicola and Botrytis cinerea are 0.33 and 0.45 mg.mL-1, respectively.

关 键 词：壳低聚糖 2-吡唑酰壳低聚糖 结构表征 抗菌活性 

分 类 号：TQ314.2[化学工程—高聚物工业]