中药骨碎补对人牙髓、牙龈、牙周膜成纤维细胞体外增殖的实验研究  

Effects of Rhizoma Drynariac on cell proliferation of human dental pulp,gingival,periodontal ligament fibroblasts cells cultured in vitro

作　　者：刘凤英[1] 许彦枝[1] 陈彦平[1] 郭晶洁[1] 高永博[1] 

机构地区：[1]河北医科大学第四医院口腔科,河北石家庄050011

出　　处：《临床和实验医学杂志》2014年第24期2012-2015,共4页Journal of Clinical and Experimental Medicine

基　　金：国家自然基金面上项目(30873289)

摘　　要：目的：探讨中药骨碎补对体外培养的人的牙髓、牙龈、牙周膜成纤维细胞增殖的影响。方法采用组织块法外培养人牙髓、牙龈、牙周膜成纤维细胞，取第4、5代培养细胞随机分为实验组和对照组，实验组加不同浓度的骨碎补，对照组只加培养液。通过HE染色观察其对牙髓、牙龈、牙周膜成纤维细胞的形态、结构及生长增殖的影响，通过MTT法和考马斯亮蓝法对实验组和对照组的细胞生长情况和总蛋白合成的检测。结果①在10-1000μg/ml浓度范围内骨碎补对人牙髓、牙龈、牙周膜成纤维细胞均有促增殖作用（ P ＜0.05），尤以100μg/ml浓度最为明显（ P ＜0.01）；②总蛋白测定结果显示在骨碎补浓度为10μg/ml时，对照组与其总蛋白含量差异无统计学意义（ P ＞0.05），实验组细胞总蛋白含量随骨碎补浓度增加高于对照组（ P ＜0.05），尤以500μg/ml骨碎补浓度组作用牙髓成纤维细胞最佳；而100μg/ml骨碎补浓度组作用牙龈、牙周膜成纤维细胞最显著。结论骨碎补在有效作用浓度范围内，其促增殖效应和总蛋白含量与骨碎补浓度呈剂量依赖关系，随着浓度的增加促增殖作用增强、总蛋白含量增加，浓度达到最大作用后，随着浓度增加作用反而呈现减弱趋势。Objective To observe the effects of Rhizoma drynariae on cell proliferation of human dental pulp,gingival,periodontal liga-ment fibroblasts cells cultured in vitro. Methods Isolated human dental pulp,gingival,periodontal ligament fibroblasts cells were cultured ac-cording to the method of tissue-explant. The fourth,fifth passage cultured cells were selected and randomly divided into experiment group and control group. The prepared Rhizoma Drynariac decoction with different concentration were added in the experiment groups. While,in control groups only culture medium were added. Which applied the Rhizoma Drynariac decoction to the cells in morphology,structure and vitro,the effects were observed by HE staining. The proliferation cells of human dental pulp,gingival,periodontal ligament were evaluated by MTT assay and the synthesis of the total proteins in the cells was measured by the method of Coomassie brilliant blue stain. Results ①The total protein con-tent of human dental pulp,gingival,periodontal ligament fibroblasts cells increased in each experiment group after adding 10-1 000 μg/ml Rhi-zoma drynariae respectively（ P 〈0. 05）. The effect of 100μg/ml was excellent（ P 〈0. 01）. ②The measurement results are displayed when the total protein concentration in Drynaria 10μg/ml. Its total protein content in the control group had no significant difference（ P 〉0. 05）. The total protein content of the experimental group increased with higher concentrations Drynaria（ P 〈0. 05）. The concentration of 500 μg/ml was excel-lent on Human dental pulp fibroblasts cells;but the 100 μg/ml was excellent on human gingival fibroblasts cells,periodontal ligament fibroblasts cells. Conclusion Drynaria effective role within the concentration range,the pro-proliferative effects,total protein concentration and dose-de-pendent Drynaria increased with increasing concentrations of total protein content,which can promote proliferation of enhanced concentration and reach a maximum effect,with the concentration

关 键 词：骨碎补 人牙髓成纤维细胞 人牙龈成纤维细胞 人牙周膜成纤维细胞 细胞增殖 总蛋白含量 

分 类 号：R780.2[医药卫生—口腔医学]