帕瑞昔布对大鼠全脑缺血再灌注损伤中学习和记忆能力的保护作用  

Role of parecoxib in protection of learning and memory ability in rats with transient global cerebral ischemia / reperfusion injury

作　　者：肖鹏冲 梅莉[2] 王召军[1] 张光燕[2] 崔建修[2] 

机构地区：[1]南方医科大学,广州市510515 [2]广东省人民医院,广东省医学科学院,广州市510080

出　　处：《实用医学杂志》2014年第24期3922-3925,共4页The Journal of Practical Medicine

基　　金：广东省科技计划(编号:2012B031800159);广东省自然科学基金(编号:S2013010011516)

摘　　要：目的:探讨帕瑞昔布对大鼠全脑缺血再灌注损伤中学习和记忆能力的保护作用。方法:雄性Wistar大鼠随机分为S组、I/R组和I/R+PA组,全脑缺血模型采用改良的Pulsinelli四血管法;再灌注72 h后连续行5 d Morris水迷宫实验评价其空间分辨学习和记忆能力;处死大鼠,灌注取脑行Nissl染色观察海马CA1区神经元的存活情况。结果:与I/R组相比,大鼠潜伏期I/R+PA组明显缩短(P<0.05),穿越平台次数和在目标象限的时间增多(P<0.05),尼氏染色CA1区神经元死亡较少。结论:帕瑞昔布对大鼠全脑缺血再灌注损伤中学习和记忆能力具有一定的保护作用。Objective To explore the role of parecoxib in protection of learning and memory ablility in rats with global cerebral ischemia/reperfusion injury. Methods Male Wistar rats were randomly divided into a sham group （S group）, a ischemia/reperfusion group （I/R group）, and a ischemia/reperfusion with parecoxib group （I/R＋PA group）. Global cerebral ischemia was induced by Pulsinelli 4-vessel occlusion. 72 h after reperfusion, Morris water maze was used to assess spatial learning and memory ability for consecutive five days. The histological changes in the hippoeampus were detected by Nissl staining. Results As compared with the I/R group, rats in S guoup and I/R＋PA group had a significantly shortened escape latency （P 〈 0.05）, and had significantly longer dwelling time in the former platform quadrant and more frequent cross-platform movement in the Morris water maze test. Conclusions Parecoxib plays a role in protection of learning and memory ability in rats with global cerebral isehemia/reperfusion injury.

关 键 词：帕瑞昔布 全脑缺血再灌注 学习与记忆 MORRIS水迷宫 

分 类 号：R741[医药卫生—神经病学与精神病学]