水稻辐射诱变突变体的种子蛋白质成分分析  

Analysis on seed protein in rice mutants induced by ^(60)Co γ-ray

作　　者：曲龙[1] 卢夏茹 李阳生[2] 韦双双[1] 袁潜华[1] 马启林[1] 

机构地区：[1]海南大学热带作物种质资源保护与开发利用教育部重点实验室,海南海口570228 [2]武汉大学杂交水稻国家重点实验室,湖北武汉430072

出　　处：《广东农业科学》2014年第22期1-4,共4页Guangdong Agricultural Sciences

基　　金：国家自然科学基金(31160259);农业部转基因作物重大专项(2009ZX08001-020B);海南大学科研基金(kyqd1045)

摘　　要：为了改良水稻品种的品质性状,利用60Coγ-射线为诱变源,对粳稻品种Koshihikari种子进行了辐照处理,通过改良的单粒种子法对种子蛋白质的含量和组成进行了SDS-PAGE和Western Blotting分析,筛选到了72个种子蛋白突变体,表明诱变后在种子蛋白的含量和组成成分方面有丰富的变异。为揭示水稻贮藏蛋白积累机制和开发新类型稻米种质提供了基础材料。In order to improve the quality traits of rice varieties, a variety named of ＂Koshihikari＂, was radiated by ^60Co γ-ray to induce mutagenesis. 72 mutants of seed storage protein were found through analyzing the content and composition of seed storage protein by SDS-PAGE and Western Blotting. These mutants had rich variations on content and composition of seed protein, and could be used as basis materials to reveal the mechanism of rice storage protein accumulation, and to develop new types of rice varieties.

关 键 词：水稻 辐射诱变 种子蛋白 突变体 

分 类 号：S511[农业科学—作物学]