马铃薯衰老相关基因的电子克隆与生物信息学分析  

Electronic cloning and bioinformatical analysis of senescence-associated genes from Solanum tuberosum

作　　者：何虎翼[1] 何新民[1] 谭冠宁[1] 李丽淑[1] 王晖[1] 何海旺[1] 唐洲萍[1] 

机构地区：[1]广西农科院经济作物研究所,广西南宁530007

出　　处：《广东农业科学》2014年第22期119-121,共3页Guangdong Agricultural Sciences

基　　金：广西科学研究与技术开发计划项目(桂科攻1222009-2F);国家马铃薯产业技术体系(CARS-10-ES15)

摘　　要：利用电子克隆获得马铃薯衰老相关基因(St SAG)的c DNA序列,并对其进行生物信息学分析。结果表明,马铃薯衰老相关基因的c DNA序列全长1 062 bp,包含一个561 bp的开放阅读框,编码186个氨基酸;St SAG的等电点为7.20,为亲水叶绿体蛋白,无信号肽,有12个磷酸化位点,在N末端有一个较短的伸展肽段。St SAG与茄科植物烟草亲缘关系最近。The c DNA sequence of senescence-associated gene（SAG） from Solanum tuberosum was acquired by electronic cloning. By using the methods of bioinformatics, we analyzed some characteristics of its encoded protein sequence. The results showed that the full-length of St SAG was 1 062 bp, which contained a 561 bp open reading frame encoding 186 amino acids. The isoelectric point of St SAG was 7.20, as a hydrophilic chloroplast protein without signal peptide. St SAG had 12 phosphorylation sites and a short extension peptide in the N terminal. The phylogenetic relationship between St SAG and Solanaceae plant was the closest.

关 键 词：马铃薯 衰老相关基因 电子克隆 生物信息学分析 

分 类 号：S532[农业科学—作物学] Q785[生物学—分子生物学]